3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) is a water-soluble ligand, is widely used to promote Cu-catalyzed alkyneazide cyclization (CuAAC), the typical bioorthogonal reaction with non-interference in living systems.
General Protocol of Labeling Alkyne-Modified Biomolecules with Fluorescent Dye Azides
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BCN-Azide Ligation
Classic Click Chemistry uses copper, Cu(I), to catalyze the 1,3-dipolar cycloaddition of an alkyne with an azide to form a 1,2,3-triazole.1,2 Sources of Cu(I) include copper(I) iodide, copper(I) bromide, copper turnings, or copper (II) sulfate (CuSO4) and a reducing agent.1 However, the ...
Bioorthogonal chemistry is a set of reactions that can take place in biological environments without affecting biomolecules or interfering with biochemical processes. For this purpose, the reaction must meet the following requirements: fast, efficient, and specific
α-Synuclein is a small molecule protein mainly found in the presynaptic terminals of the central nervous system. It consists of 140 amino acids and has a molecular weight of about 19 kDa.
To a TEOS (2.5 mL) solution in 115 mL of dried ethanol, 3.75 mL of aqueous ammonium hydroxide solution (14.6 M) and 3.75 mL of water were added while stirring. After 12 h of stirring, silica nanoparticles were isolated by centrifugation at a speed of 15,000 rpm and the supernatant ...
PEGylated nanoparticles are powerful new synthetic tools with differing properties to their nanoparticle precursors. Polyethylene glycol (PEG) can be coated around nanoparticles to alter their properties and offer fascinating new biomedical applications. In particular, the bioconjugation of ...
