Refolding experiments after inclusion body proteolysis
When proteins are expressed at high levels in E. coli and other expression hosts, it is common to find that most of them are insoluble and exist in insoluble fo
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Sodium Azide Removal Protocol
Sodium azide is a commonly used preservative to prevent bacterial and fungal contamination in antibody solutions. However, sodium azide is cytotoxic, interferes with antibody coupling reactions, and inhibits the activity of enzymes such as horseradish peroxidase (HRP).
Bielschowsky Silver Staining Technique
Bielschowsky silver staining is a classic silver staining technique, which is mainly used in the pathological study of nervous system tissues, especially in showing structures such as neuronal axons, neurofibrillary tangles and senile plaques.
Deproteinization Using Perchloric Acid Precipitation Protocol
In biological sample analysis, the presence of proteins often interferes with small molecule detection, and some enzyme activities may affect the analysis results. Perchloric acid (PCA) precipitation has become a widely used method due to its ability to efficiently remove proteins and its ...
Flow Cytometry Using Methanol Permeabilization
Flow cytometry is a powerful technique that is often used to analyze surface and internal antigens of cells. When detecting intracellular antigens, cells usually need to be fixed and permeabilized.
Guidelines for Using Isotype Control Antibodies
Isotype control antibody is a negative control reagent that has the same immunoglobulin class, subclass, and light chain type as the research antibody (experimental antibody) but does not bind to the target antigen.
Multiplex Immunohistochemistry Protocol
Multiplexed immunohistochemistry (mIHC) is an in situ multiple labeling method based on tyramide signal amplification (TSA) technology, which can achieve simultaneous detection of multiple targets (usually 6-8 types) on a single tissue section. Its core principle is to covalently bind the target ...
Tris Glycine Buffer 5x
Tris Glycine Buffer 5x
Azido-PEG3-Maleimide
Azido-PEG3-Maleimide