3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Broadpharm Antibody Conjugation Kits contain most popular sulfonated reactive dyes which are most suitable for the labeling antibodies and other sensitive proteins. The kits provide optimized reagents for labeling antibodies having 2.5-3 labels per antibody, and desalting columns for purifying ...
The BroadPharm Dye Maleimide Antibody Labeling Kits contain sulfonated maleimide dyes which label thiolcontaining proteins, including antibodies. The kit provides optimized reagents for labeling antibodies having 2.5-3 labels per antibody and desalting columns for purifying the labeled molecule. ...
Maleimide-PEG modified drug can be conjugated to the antibody at the pH 7.5 buffer solution contained 50 mM sodium phosphate, 50 mM sodium chloride and 2 mM EDTA The disulfide bridges in the antibody were then reduced with various molar equivalents of DTT at 30 ̊C for 30 min to afford different ...
Biotin Quantitation Kit
Ellman’s reagent, 5,5′-dithio-bis-(2-nitrobenzoic acid), also known as DTNB, is a water-soluble compound reacts with free sulfhydryl groups in solutions at slightly alkaline condition to yield a mixed disulfide and highly chromogenic 5-nitro-2- thiobenzoic acid (TNB) with molar extinction ...
Cell Viability Assays
ChIP is a powerful tool that uses isolated chromatin and antibodies against the target antigen to determine whether the target binds to a specific DNA sequence or to map its distribution across the genome (ChIP-seq).
MTT colorimetry is a detection method based on cell metabolic activity. It is widely used in cell biology and pharmacology research to evaluate cell proliferation, cell activity, cytotoxicity, and drug screening.
The DNA of apoptotic cells is cut into fragments of about 200 base pairs under the action of caspase-activated nuclease (CAD), forming DNA ladders. By detecting DNA fragmentation during apoptosis, the degree of apoptosis can be evaluated.
