Cell freezing and resuscitation experiments
Cell freezing and resuscitation can be (1) used for biological preservation, (2) used for stem cell research in medicine, and (3) used for passaging culture.
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Mouse peritoneal macrophage culture experiment
Mouse peritoneal macrophage cultivation can (1) phagocytosis and removal of foreign substances; (2) secretion of bioactive substances; (3) to bionic fully degra
MTT assay for cytotoxicity and proliferation
The MTT assay is a common method for cell growth detection by MTT (chemical name 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide, trade name thiazolyl
platelet
Single-cell spreading plate is to add appropriate amount of complete medium into each well of cell culture plate in advance, shake well to make the medium infil
Experimental purification of PCR products with dUTP
The incorporation of dUTP into the replicon during PCR amplification is one of the most efficient and widely used methods for purifying PCR products (Longoetal.
Purification of sequencing reaction products
Purification of the sequencing reaction product prior to electrophoresis is essential to remove excess, unbound dye terminators. Centrifugation columns can be a
Primer extension experiment
Primer extension analysis was used to determine the location and quantify the amount of the 5'-end of a specific RNA. To end the labeled oligonucleotide hybridi
Restriction fragment length polymorphism (RFLP) analysis
Restriction fragment length polymorphism (RFLP) is one of the most important analytical methods in molecular biology and is mainly used to (1) detect DNA sequen
Precision amplification experiments for large fragments
Amplify a ~5kb fragment with 10 different primer pairs, the template DNA is the same, the reaction system is 50ul, and the control has no template and contains