PCR-SSCP technology experiment
Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) technology can be used for: (1) screening and detection of oncogenes and oncogenic
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PCR product purification assay
A method is described for the purification of PCR products using an AmiconMicrocon-PCR filter (Millipore) that efficiently removes redundant dNTP and primers. T
PCR product quantification
Accurate quantification of the template prior to sequencing is critical. Depending on the amount of DNA used, several different methods are available. Two other
Sequencing reaction experiments
To get the right signal intensity, adjust the amount of DNA according to the size of the DNA fragments. For ABI PRISM Big Dye Terminator Ready Reaction mix (App
PCR amplification of high GC content fragments
The following program describes in detail the PCR amplification of high GC content antique fragments. This experiment was derived from PCR Laboratory Guide (Sec
Genomic DNA purification from whole blood Experimental methods
The Wizard Genomic DNA Purification Kit for isolation of genomic DNA from whole blood (10 ml) is based on a four-step procedure. The first step of the purificat
Preparation of RNA by ultracentrifugation with guanidine isothiocyanate/cesium chloride
This method is derived from a modification of the protocol developed by Chirgwin et al. (1979). The reagents specified for Chirgwin et al. are commonly used in
Purification of total RNA using the Marligen Total RNA Rapid Purification System
The Rapid Total RNA System by Marligen Biosciences extracts RNA from a variety of samples with very consistent yield and quality. This experiment was derived fr
Preparation of RNA from plant tissues using Trizol
The following protocol is a modified version of the Trizol protocol for RNA isolation from plant tissues in which a high-salt isopropyl fermentation precipitati