High performance reversed-phase liquid chromatography
High performance reversed-phase liquid chromatography can be applied to: Separate non-polar, polar or ionic compounds.
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Expression and purification experiments of glutathione S-transferase fusion protein
The pGEX vectors can be used to express and purify peptides (including short peptides) used as immunogens and biochemical and biological reagents, or to constru
Determination of the activity of myocardial sarcoplasmic reticulum calcium-triphosphate adenosine monophosphatase
Cardiac sarcoplasmic reticulum calcium-ATPase (SERCA2a) plays a major role in the regulation of calcium homeostasis in cardiomyocytes, and studies on the gene t
affinity chromatography
The affinity molecules with special structure are made into solid-phase adsorbent and placed in the chromatography column, when the protein mixture to be separa
Protein recovery assay
Recovery test is a type of "control test". When the analyzed sample is complex and not completely clear, a known amount of the measured component is added to th
Capture experiments of interacting proteins
Capture assays for interacting proteins are subjected to two sequential bulk yeast plate screening processes. Saccharomyces cerevisiae contains the lexA fusion
Characterization of decoy proteins
The first step in capturing interacting proteins is to construct a decoy protein, which is a fusion of LexA to the target protein. A series of control experimen
Probe synthesis experiments
A probe is a small fragment of single-stranded DNA or RNA (approximately 20 to 500 bp) that is used to detect a nucleic acid sequence complementary to it. Doubl
Extraction of myocardial sarcoplasmic reticulum calcium-triphosphate adenosine monophosphatase
Cardiac sarcoplasmic reticulum calcium-ATPase (SERCA2a) plays a major role in the regulation of calcium homeostasis in cardiomyocytes, and studies on the gene t