Immunofluorescence localization experiments of nuclear proteins
Immunofluorescence localization of nuclear proteins can be applied to (1) localize intracellular distribution of nuclear proteins and (2) viral studies.
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Immunoscreening assay
A technique for selecting a target from a population based on the principle of antigen-antibody interaction. For example, antibodies are used to detect proteins
Molecular exclusion high performance liquid chromatography experiments
Molecular exclusion chromatography, also known as gel chromatography, is the use of different molecular sizes of the separated substances lead to different degr
Metallothionein Measurement Experiment
Metallothionein is a short cysteine-rich peptide produced by microorganisms and plants as a metal-binding protein with a high affinity for a wide range of heavy
Electrophoretic separation and identification of proteins on cellulose acetate membranes
The isoelectric points of various proteins in mixed protein samples are different, and the amount of electrostatic charge carried by various proteins is differe
Analysis of N-terminal amino acids of proteins by dansulfonylation method
The reaction of α-amino group of protein with dansulfonyl chloride (DNS-Cl is a fluorescent substance) produces DNS-protein, which can be hydrolyzed to produce
Molecular weight determination of proteins by gel filtration chromatography experiment
Gel filtration is also known as exclusion chromatography, molecular sieve chromatography and gel chromatography. The purpose of this experiment is to understand
Separation of nucleotides by chromatography on ion exchange columns
In this experiment, yeast RNA was used as the material, and the RNA was hydrolyzed into mononucleotide by alkali, then separated by ion exchange column chromato
The effect of substrate concentration on catalytic reaction rate and the determination of Mie constant Km and maximum reaction velocity Vmax
In this experiment, the Km value of sucrase was calculated by using the amount of products (glucose and fructose) formed by sucrase hydrolyzing different concen