Salt chromatographic graded separation and gel chromatographic desalting of proteins
The purpose of this experiment is to understand the basic principles and operation of salting out graded separation of proteins; to understand the basic princip
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Expression and purification experiments of thioredoxin fusion protein
A gene fusion expression system using the E. coli trxA gene product-thioredoxin as a fusion partner is particularly suitable for the expression of high yield so
Experiments on the preparation of yeast sucrase
Sucrase (inver tase) (β-D-fructofuranoside fructohydrolase) (EC. 3 . 2 . 1 . 26 ) catalyzes the hydrolysis of α-fructofuranoside bonds in non-reducing sugars
Metabolic labeling experiments of recombinant proteins
Since host protein synthesis is essentially terminated when recombinant proteins are expressed, in vivo metabolic labeling is a sensitive method for detecting r
Phosphate amino acid analysis experiment
It is of interest to identify phosphorylated amino acid residues in proteins. Phosphorylation occurs at serine, threonine, and methionine in proteins, and the i
Experiments to determine peak protein production
Protein is the material basis of life, without which there is no life. It is therefore a substance that is closely associated with life and with all forms of vi
Electrotransfer experiments on proteins
Many membranes can be used as solid-phase supports for protein electrotransfer, such as diazotized cellulose membranes (DPT, DBM), DEAE-cellulose membranes, nyl
Cyanogen bromide cleavage assay of calmodulin
This experiment describes the cyanogen bromide cleavage process of calmodulin. This experiment is from the Experimental Guide for Protein Purification and Ident
Serum lipoprotein agarose electrophoresis assay
After serum lipoproteins are stained with Sudan black B and electrophoresed in pH 8.6 barbiturate buffer with agarose, the lipoproteins can be divided into diff