Preparation of monoclonal antibodies against phosphopeptides.
This experiment provides steps starting from inoculation of candidate hybridoma clones into a 96-well culture plate all the way through to isolation of a hybrid
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Protein precipitation technology
Although many different precipitation methods have been used over the past 100 years, AS precipitation has been the most widely used, especially for acidic prot
Determination of phosphorylated amino acid positions in peptides
If the sample volume is insufficient for direct sequencing, the peptide can be degraded by manual Edman degradation to determine the location of the phosphoryla
Protein elution experiments in gels
SDS-PAGE has proven to be an extremely useful analytical method in determining the number and size of peptides in a sample. It also has the ability to separate
Analyzing peptide separation experiments
CE is particularly useful for separating specific peptides from complex mixtures such as proteolytic products. The following protocol describes the use of the P
Detection of specific amino acids in phosphopeptides by secondary discriminatory digestion assay
More information about phosphopeptides can be obtained by digestion with sequence-specific proteases or cleavage with site-specific chemicals. From The Compact
Hydroxyapatite columns for protein chromatography experiments
Hydroxyapatite (HA) is a calcium phosphate-based hydroxylate that has been used extensively in the chromatographic separation of proteins, mainly in 1991-2009,
Identification, production and use of polyhydroxy-reactive monoclonal antibodies - for immunoaffinity chromatography
All forms of affinity chromatography require the formation of a specific interaction between two components by which one of the components can be purified. Immu
Ca2+/calmodulin-dependent kinase analysis assay
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when