Selection of suitable methods for recombinant protein expression
Recombinant proteins are important tools for studying biological processes. Expression systems are needed for their preparation. The choice of a suitable expres
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
A proteomic approach to the risk assessment of peroxisome proliferating contaminants in the marine environment
Pollution of the marine environment is a growing concern because it can affect humans and other natural species. The application of proteomic methods to monitor
Proteomic analysis of AtT20 pituitary cells as a model for neuroendocrine peptidergic system interference
Environmental contaminants can affect many intracellular enzyme activities. The effects of enzyme inhibitors on the proteome can be determined using bidirection
Labeling experiments for protein expression
Most proteins are prepared using recombinant expression techniques. During the cloning process, additional residues or tags can be added to the N- or C-terminus
Maintenance of protein stability
This method is mainly used to maintain the stability of proteins.
Isoelectric spot focusing for protein separation
Proteins can be separated on coated or uncoated capillary columns, and the choice of separation protocol depends on the specific properties of the target protei
Protein gel staining assay
This chapter describes electrophoretic-based protein assays, focusing on the current methods for total protein assay, post-translational modification (PTM) assa
Purification experiments of membrane proteins
Membrane proteins play a key role in biological processes. They are involved in cell-cell and cell-matrix interactions, cell organelle and cell formation, trans
Protein purity assay
No method can directly quantify the purity of a protein sample. Typically, protein purity determination involves evaluating the level of specific impurities or