Capillary electrophoretic analysis of proteins
The main features of capillary electrophoresis are high column efficiency (N＞105-106), short analysis time, low consumption of sample volume and reagents, mult
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Tyrosine kinase analysis assay
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when
Experiments for direct analysis of kinases in gels
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when
Determination of protein concentration by Lowry's assay
The Lowry method is a standardized, rapid method for protein quantification and has been widely used to remove interfering substances by protein precipitation p
Chemical degradation of fusion proteins
The use of gene fusion expression systems to express exogenous genes in E. coli has become increasingly popular. The reason for this is largely attributed to th
Preparation of affinity columns
This section will focus on the solid-phase technique of ligand solidification on agarose gels activated with cyanogen bromide. The vast majority of ligands, suc
Solubilization and replication experiments of inclusion body proteins
Although inclusion bodies are mixtures of unnatural forms of expressed proteins, the peptide chain itself is intact, or the primary structure is correct. One ad
Fusion Protein Enzymolysis Assay
The use of gene fusion expression systems to express exogenous genes in E. coli has become increasingly popular. The reason for this is largely attributed to th
Cyclic nucleotide-dependent protein kinase analysis assay
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when