3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Dialysis utilizes a semipermeable membrane with a defined molecular weight cut-off (MWCO). Driven by concentration gradients in a large external volume, small molecules diffuse out of the sample to achieve desalting, removal of small-molecule inhibitors/dyes, and buffer exchange. This document ...
To efficiently remove endotoxins (lipopolysaccharides, LPS) from aqueous protein samples using the temperature-dependent phase separation property of Triton X-114, while retaining the target protein in the aqueous phase.
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