3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Biological processes are complex and determined by networks of interactions, called interactome networks, which include protein-protein interactions. The protein interactome is elemental to cellular function, but our full understanding of it is incomplete. One assay that has helped us begin to ...
IPTG Induction and Extraction of Proteins Modified
IPTG – 100mM (100X) Stock Solution
Blue-white screening of bacterial colonies is a popular and effective molecular biology tool often used to detect recombinant bacteria in cloning experiments. Central to this technique is the enzymatic activity of β-galactosidase, a tetrameric enzyme encoded by the lacZ α gene in E. coli that ...
Immunofluorescence Protocol
Protein blotting (WB) is widely used to analyze the expression of specific proteins in cell or tissue extracts. Western blot experiments are time-consuming, and their success has a significant impact on research progress.
Experimental Steps for Methanol Permeabilization & Triton X-100 Permeabilization
The detection of cell surface proteins mainly targets antigens on the cell membrane. Antibodies can directly bind to antigens on the cell surface, so fixation and permeabilization treatment are usually not required. The experimental operation is relatively simple, and the main focus is on the ...
CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/Insitu hybridization-compatible Tissue hYdrogel) is a revolutionary tissue clearing and staining technology that enables three-dimensional imaging and analysis of intact biological tissues by combining hydrogel ...
