Step-by-step protocol for Cy5 PEG Biotin conjugation, fluorescence imaging, and streptavidin-based detection. Optimized for microscopy and flow cytometry applications.
Fluorescent labeling
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Explore the spectral properties, labeling protocols, and research applications of 6-Carboxytetramethylrhodamine (6-TAMRA) for fluorescence-based assays.
Phycocyanin (PC) is a deep-blue, water-soluble pigment protein that is isolated and purified from algal biomass such as Spirulina. It is typically supplied as a blue powder or granules, readily soluble in water but insoluble in alcohol and oils.
Microparticles have exhibited extensive utility across various fields including medicine, biochemistry, colloid chemistry, and aerosol research. Their applications encompass chromatographic separation media, serving as supports for immobilized enzymes, and functioning as spacers in liquid ...
Fluorescein isothiocyanate (FITC) is a green fluorescent dye widely used in biological labeling and imaging techniques. Polysaccharides are important biological macromolecules involved in various biological processes and functions. By labeling polysaccharides with FITC, their visualization and ...
Techniques often explored alongside immunological experiments.
