GFP Mouse mAb - Primary antibody, specific to GFP, Mouse IgG1

Cat. No.: Ab105284

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, 1.0 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Species independent

Isotype

Mouse IgG1

Clone Number

3A10

Conjugation

Unconjugated

Overview

Mouse anti GFP Antibody, Monoclonal (3A10), could be used for WB, IP and so on.

Specifications

Product Name

GFP Mouse mAb - Primary antibody, specific to GFP, Mouse IgG1

Synonyms

GFP antibody | Green fluorescent protein antibody | eGFP | Enhanced Green Fluorescent Protein | GFP | GFPuv | green fluorescent protein (gfp) | Green Fluorescent Protein | GFP Tag antibody | Anti- GFP Tag antibody

Specifications & Purity

ExactAb™, Validated, Carrier Free, High performance, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca 2+ -activated photoprotein aequorin. Subunit struct

Host species

Mouse

Specificity

GFP

Immunogen

Synthetic peptide GFP.

Positive Control

WB: HEK293T whole cell lysate transfected with pEGFP-C3-ATG3, HEK293T whole cell lysate transfected with pEGFP-C3-ATG12. IP: HEK293T transfected with pEGFP-C3-ATG3#PPL00361-2a.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, High Performance, Validated

Isotype

Mouse IgG1

SDS-PAGE

150 kDa

Purification Method

Antigen affinity purified

Concentration

1.0 mg/mL

Note

This product is about to be sold out and taken down. The replacement product is Ab169015; Ab186981; Ab186982

Storage and Shipping

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: GFP Mouse mAb (Ab105284)

GFP Mouse mAb (Ab105284) - Western Blot
All lanes: GFP Mouse mAb (Ab105284) at 1/10000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 27 kDa
Observed band size: 29 kDa
Exposure time: 2.3 s

Western Blot: GFP Mouse mAb (Ab105284)

GFP Mouse mAb (Ab105284) - Western Blot
All lanes: GFP Mouse mAb (Ab105284) at 1/10000 dilution
Samples: Recombinant GFP Protein (rp156636) at 20 ng per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (138040) at 1/30000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 24.3 s

Application

• Application: Dilution info
• WB:

  1/5000 – 1/10000 ;

• IF/ICC:

  1/100-1/500 ;

Frequently Asked Questions

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GFP Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.