Topic

Primary antibody

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

7 resources · protocols, FAQs & articles
article

An isotype control antibody appears like a typical antibody but it does not attach itself to a specific target protein like the primary antibody used in the experiment.

Updated Jun 2025·33 min read
article

Choosing monoclonal or polyclonal antibodies? A single specific antibody produced by one type of B cell is called a monoclonal antibody. Monoclonal antibodies bind to a single specific antigenic determinant in a targeted manner, like a missile hitting a target with precision.

Updated Jun 2025·33 min read
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Since the first publication describing Western blotting, this immunodetection technique has been widely used to identify specific proteins in complex mixtures extracted from cells or tissues.

Updated Jun 2025·33 min read
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IgG, the key factor of Humoral immunity response, is the main immunoglobulin in blood, lymph, cerebrospinal fluid and peritoneal fluid. IgG accounts for approximately 75% of total serum immunoglobulins and is the only immunoglobulin that can penetrate the human placenta. It is primarily ...

Updated Jun 2025·33 min read
Related topics

Techniques often explored alongside immunological experiments.

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