Sodium azide is a commonly used preservative to prevent bacterial and fungal contamination in antibody solutions. However, sodium azide is cytotoxic, interferes with antibody coupling reactions, and inhibits the activity of enzymes such as horseradish peroxidase (HRP).
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Recombinant antibody
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
5 resources · protocols, FAQs & articles
Whole mount staining is the staining of small pieces of tissue-usually embryos-without sectioning.
The Interleukin (IL) family is a group of cytokines and development factors mainly derived from white blood cells, which play a key role in coordinating the immune response.
Primary antibodies are antibodies that bind directly to the target protein and have a variable antibody region that recognizes the protein's epitope.
IgG, the key factor of Humoral immunity response, is the main immunoglobulin in blood, lymph, cerebrospinal fluid and peritoneal fluid. IgG accounts for approximately 75% of total serum immunoglobulins and is the only immunoglobulin that can penetrate the human placenta. It is primarily ...
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Techniques often explored alongside immunological experiments.