ExactAb™, Validated, Recombinant, High performance, 0.05 mg/mL ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human ACTA2 Antibody, Recombinant (RR645), could be used for WB, IHC, Flow, IF, ICC and so on.
Application:
WB: 1/1000 - 1/2000
IHC: 1/2000 - 1/4000
Flow: 1/20 - 1/100
IF/ICC: 1/80 - 1/250
Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant ACTA2 Antibody (Ab088446) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 44 kDa
Exposure time: 80.0 s
Recombinant ACTA2 Antibody (Ab088446) - IF
IF analysis of ACTA2 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant ACTA2 Antibody (Ab088446) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant ACTA2 Antibody (Ab088446) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta tissue labelling ACTA2 with Recombinant ACTA2 Antibody (Ab088446) at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant ACTA2 Antibody (Ab088446) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ACTA2 Antibody (Ab088446) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant ACTA2 Antibody (Ab088446) (1/100 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Green) was used as a control.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000 |
| IF/ICC | 1/80 - 1/250 |
| Flow Cytometry | 1/20 - 1/100 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0911322 | Certificate of Analysis | Jul 10, 2026 | Ab088446 |
| ZJ23F0901100 | Certificate of Analysis | Sep 18, 2023 | Ab088446 |
| ZJ23F0901101 | Certificate of Analysis | Sep 18, 2023 | Ab088446 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →
