Recombinant Cdc23 Antibody - Primary antibody, specific to CDC23, Rabbit IgG

Cat. No.: Ab095583

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.43 mg/mL

Application

WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human Cdc23 Antibody, Recombinant, could be used for WB and so on
Application:
WB: 1/500-1/1000
Protein Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Specifications

Product Name

Recombinant Cdc23 Antibody - Primary antibody, specific to CDC23, Rabbit IgG

Synonyms

Cell division cycle protein 23 homolog | CDC23_HUMAN | APC8 | CUT23 | ANAPC8 | Anaphase-promoting complex subunit 8 (APC8) | Cyclosome subunit 8 | ANAPC8 antibody | Anaphase promoting complex subunit 8 antibody | Anaphase-promoting complex subunit 8 antib

Specifications & Purity

ExactAb™, Validated, Recombinant, 0.43 mg/mL

Host species

Rabbit

Specificity

CDC23

Immunogen

A synthetic peptide derived from human Cdc23/APC8 (AA 510-575)

Positive Control

WB: Hep G2, Jurkat, K562, HEK293, HeLa, NIH/3T3 and C6 cell lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

0.43 mg/mL

Product Line

Biological Products

Storage and Shipping

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant Cdc23 Antibody (Ab095583)

Recombinant Cdc23 Antibody (Ab095583) - Western Blot
All lanes: Recombinant Cdc23 Antibody (Ab095583) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 69 kDa
Observed band size: 65 kDa
Exposure time: 27.2 s

Application

Application

Dilution info

WB

1/500-1/1000

Associated Targets(Human)

CDC23 Tbio Cell division cycle protein 23 homolog (0 Activities)

Discover Target: CDC23

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Manufacturer-validated applications and recommended dilutions: Not specified for this item; refer to CoA/Spec Sheet.

General protocol guidance (use as a starting point only)

• Western blot: Begin at 1:1,000 primary dilution in 5% BSA/TBST; incubate 1 h at RT or overnight at 4°C. Titrate ±10× based on signal/background.
• Immunofluorescence/ICC: Start at 1–5 µg/mL or 1:200–1:500 in blocking buffer; fix/permeabilize per cell-type requirements.
• IHC: Trial dilutions 1:100–1:500 with appropriate antigen retrieval; include positive/negative controls.
• Immunoprecipitation: 1–5 µg antibody per mg lysate protein; optimize lysis buffer to preserve APC/C complexes for Cdc23.
• ELISA: If using as capture/detection, determine coating concentrations (0.5–2 µg/mL) and validate with standard curves.

Controls and validation

• Include isotype controls and secondary-only controls.
• Use orthogonal validation (e.g., siRNA/CRISPR knockout) to confirm band/spot identity.

Important

• The above are general suggestions. Adopt conditions from the CoA when available and perform pilot optimization for your sample type.

Biological Roles

Target biology overview (literature; not product claims)

• Cdc23, also referred to as APC8/Apc8 in many organisms, is a core subunit of the anaphase-promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase governing metaphase–anaphase transition and mitotic exit by targeting substrates (e.g., securin, cyclins) for proteasomal degradation.
• Functional context: Cdc23 contributes to APC/C assembly and substrate recognition via interactions with other TPR (tetratricopeptide repeat)-containing subunits (e.g., Cdc27/Apc3). APC/C activity is regulated by co-activators (Cdc20, Cdh1) and checkpoint pathways. Perturbation of APC/C components can alter cell-cycle progression.
• Localization: APC/C components are typically cytoplasmic and nuclear, with cell cycle–dependent dynamics (literature). Specific localization of Cdc23 may vary by species and cell type.

Relevance to experimental design

• Epitope considerations: Structural participation of Cdc23 in large complexes can mask epitopes; denaturing conditions (WB) may reveal epitopes that are less accessible in native assays. Crosslinking in IP can stabilize transient interactions.
• Species orthology: Cdc23/APC8 is conserved across eukaryotes; ensure species reactivity matches your sample. Species reactivity for this SKU is not specified; confirm on the CoA.

Important

• The above describes biology of the antigen, not performance of this antibody. For validated applications, species, and epitope details specific to Ab095583, refer to the product’s CoA/Spec Sheet.

Buffer Applications

This product is an antibody reagent and is not itself a buffer system.

General buffer guidance for immunoassays (literature; not item-specific)

• Common running/assay buffers: PBS or TBS at pH ~7.2–7.6 for dilutions and washes; 0.05–0.1% Tween-20 often used to reduce nonspecific binding.
• Blocking buffers: 1–5% BSA, nonfat dry milk, or serum depending on assay (WB/ELISA/IHC). Optimize to balance background vs signal.
• Antigen retrieval (IHC): Citrate (pH ~6) or Tris-EDTA (pH ~9) heat-induced retrieval buffers chosen based on epitope stability; determine empirically for Cdc23.
• Storage/dilution compatibility: Verify preservative content and carrier proteins in the supplied formulation before selecting sensitive downstream applications (e.g., live-cell).

Item-specific buffer composition of Ab095583 is not specified; refer to the CoA/Spec Sheet for exact formulation and any compatibility constraints.

Green Alternatives

While “green alternatives” typically refer to solvents/reactants, antibody reagents also afford opportunities to reduce environmental impact.

Practical sustainability considerations (general guidance)

• Sample economy: Use micro-volume workflows (e.g., microplate ELISA, capillary Western systems) to reduce reagent consumption and waste.
• Buffer selection: Choose phosphate- or Tris-based buffers prepared from concentrated stocks to minimize packaging waste; avoid excess single-use plastics.
• Preservative handling: If sodium azide is present (not specified for this item), segregate azide-containing aqueous waste and avoid discharge to copper/lead plumbing.
• Shipping and cold chain: This product is shipped with ice pads in an ice chest. Consolidate orders when possible to reduce shipping materials and emissions.
• Storage efficiency: Aliquot to minimize freeze–thaw and prevent wastage due to degradation.

Comparison (general)

• Polyclonal vs recombinant: Recombinant antibodies (this item) generally provide improved lot-to-lot consistency, reducing repeat experiments and waste associated with variability.
• Ready-to-use formats: Where available, RTU or concentrated carrier-free formats can reduce auxiliary reagents and simplify workflows; formulation for this SKU is not specified.

Note: No changes to the product’s recommended storage or handling should be made solely for “green” reasons if they risk quality or performance. Always defer to the Storage & Reconstitution and SDS guidance.

Pharmaceutical Uses

This is a research-grade recombinant antibody intended for laboratory research use only.

• Not for diagnostic, clinical, or therapeutic use.
• No pharmacopeial status, GMP grade, or clinical formulation details are provided for this item.

General context (for awareness only; not product claims)

• Antibodies can serve as analytical reagents in bioprocess monitoring, impurity assays, or characterization workflows in pharmaceutical R&D and QC. If such usage is intended, verify that the formulation (e.g., absence of sodium azide or carrier proteins) is compatible with your analytical platform.
• For any application in regulated environments, obtain full documentation (CoA, lot traceability, impurity profiles). This product is not specified to meet such requirements.

Conclusion

• Pharmaceutical excipient or therapeutic roles do not apply to this SKU. Use strictly within research settings according to institutional and regulatory guidelines.

Physical Properties

Item-specific physical properties

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer composition, protein amount: Not specified for this item; refer to CoA/Spec Sheet.
• pH, excipients/preservatives (e.g., sodium azide, BSA, glycerol): Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General/literature context for antibodies (for planning purposes only; not specifications)

• Typical IgG molecular weight: ~150 kDa; Fab ~50 kDa; scFv ~25–30 kDa.
• Solubility: Antibodies are water-soluble proteins in physiological buffers (e.g., PBS, TBS) when formulated appropriately; solubility and stability depend on pH, ionic strength, and excipients (literature).
• Isoelectric point (pI): Often in the range pH 6–9 depending on sequence and glycosylation (literature). Actual pI for this reagent is not specified.
• Thermal sensitivity: Proteins denature with heat; freeze–thaw cycles can cause aggregation.
• Light sensitivity: Many proteins are moderately light-sensitive; minimize prolonged exposure to strong light during storage and handling.

Practical notes

• Viscosity and density of antibody solutions depend on concentration and formulation; do not assume water-like values at high concentrations.
• Avoid foaming and vigorous vortexing to minimize aggregation.
• For precise handling (e.g., molar dosing, coating), obtain the exact concentration and formulation from the CoA and use spectrophotometry at 280 nm (with the product-specific extinction coefficient, if provided).

Quality & Grades

Item-specific quality descriptors

• Grade/Purity: Recombinant, ExactAb™, Validated (as listed by manufacturer).

What these terms generally indicate (general/literature context)

• Recombinant: Antibody produced via recombinant expression, enabling defined sequence control, batch-to-batch consistency, and reduced background from hybridoma drift. It also allows for engineered formats (isotype switching, Fc mutations) when specified.
• ExactAb™: Brand/line designation indicating a defined, sequence-specific recombinant antibody offering enhanced lot-to-lot reproducibility. Exact attributes (isotype, sequence, affinity) for this SKU are not specified; refer to CoA/Spec Sheet.
• Validated: Indicates the supplier has performed application- or target-specific validation. Exact validated applications, species reactivity, and performance metrics are not listed here; consult product documentation for the validation dataset and recommended dilutions.

Quality considerations and practical guidance

• Lot-to-lot consistency is typically superior for recombinant antibodies; still verify performance in your assay with small-scale pilot tests.
• If your workflow is sensitive to excipients (e.g., mass spectrometry, live-cell work), review the formulation and select azide-free or carrier-free variants if available. Formulation for this item is not specified.
• For regulated workflows, request CoA including concentration, purity assessment (e.g., SDS-PAGE), endotoxin levels, and host cell protein/DNA residuals, if applicable.
• Maintain a frozen archive of aliquots to reproduce results across projects and to avoid freeze–thaw cycles as indicated under Storage.

Reaction & Applications

This item is a recombinant antibody intended for immunodetection/affinity applications rather than chemical synthesis.

Typical research applications for antibodies (general guidance; not item-specific validation)

• Western blot (WB): Detection of denatured target proteins in lysates after SDS-PAGE and transfer. Optimize antibody dilution, blocking reagent, and secondary antibody.
• Immunoprecipitation (IP)/Co-IP: Enrichment of target proteins and complexes; verify antibody compatibility with native vs denatured conditions.
• Immunofluorescence (IF)/Immunocytochemistry (ICC): Subcellular localization in fixed cells; optimize fixation (PFA vs methanol) and permeabilization.
• Immunohistochemistry (IHC): Detection in tissue sections; antigen retrieval conditions (heat/enzymatic) and blocking steps are critical.
• ELISA: Quantitative assays using capture/detection formats; requires knowledge of epitope accessibility.

Target context (literature)

• Cdc23 is a component of the anaphase-promoting complex/cyclosome (APC/C; also known as APC8/Apc8), involved in ubiquitin-mediated proteolysis during cell cycle transitions. Epitope accessibility may differ between native and denatured states; optimization may be needed across applications.

Important

• The manufacturer’s validated applications for this SKU are not listed here. Treat the above as general possibilities and confirm suitability in your assay system. Start with small pilot experiments to establish working dilutions and conditions.

Reaction Conditions

Classical chemical reaction conditions do not apply to antibody use. Instead, consider immunoassay operating conditions.

General immunoassay conditions (literature; not item-specific)

• Western blot: Primary incubation typically 1–16 h at 4–25°C; blocking with 3–5% BSA or milk; wash with TBS-T/PBS-T; secondary HRP or fluorescent conjugates as appropriate.
• Immunofluorescence/ICC: Primary incubation 1–16 h at 4–25°C; fixation with paraformaldehyde or methanol depending on epitope; permeabilization with 0.1–0.5% Triton X-100 or saponin as required.
• IHC: Heat-induced epitope retrieval in citrate (pH 6) or Tris-EDTA (pH 9); primary incubation 1–16 h; appropriate blocking to reduce background.
• ELISA: Coating antigen overnight at 4°C; blocking; primary incubation 1–2 h; stringent washes.

For Cdc23 specifically (literature)

• Being part of the APC/C complex, epitope accessibility may vary. For IP, consider mild lysis buffers preserving complexes (e.g., NP-40 or digitonin-based) and optional crosslinkers.

Important

• The validated applications, optimal dilutions, and incubation parameters for Ab095583 are not specified. Use the above as starting points and optimize in pilot experiments. Always consult the product’s CoA for any vendor-recommended conditions.

Safety & Handling

Hazard information (item-specific)

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• Research use: For research use only (not for diagnostic, clinical, or therapeutic use).

General safety guidance for antibodies and protein reagents (literature/best practice)

• PPE: Wear lab coat, gloves, and safety glasses. Use biological safety cabinet if handling potentially biohazardous materials (e.g., cell lysates) in conjunction with the antibody.
• Inhalation/ingestion/skin contact: Low acute hazard expected for purified antibodies; nonetheless, avoid ingestion and inhalation. Wash exposed skin with soap and water. Seek medical attention if irritation persists.
• Preservatives: Some antibody formulations may contain sodium azide or other biocides. If present, azide is acutely toxic and may react with copper/lead plumbing—collect waste appropriately. This product’s preservative content is not specified; consult SDS/CoA before disposal.
• Incompatibilities: Avoid strong oxidizers, proteases, and extreme pH that can denature proteins. Do not mix with bleach if sodium azide is present.
• Special risks: Repeated freeze–thaw can cause aggregation and loss of activity; aliquot upon first thaw.

First-aid overview (consult SDS for authoritative instructions)

• Eyes: Rinse cautiously with water for several minutes; remove contact lenses if present and easy to do.
• Skin: Wash with soap and water.
• Inhalation: Move to fresh air; seek medical attention if symptoms occur.
• Ingestion: Rinse mouth; do not induce vomiting; seek medical advice.

Solvent Selection

This product is a protein antibody reagent and is not used as a solvent. Conventional solvent selection (polarity, dielectric constant, miscibility) is not applicable.

Practical handling media (general guidance)

• Typical assay buffers: Phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), often with 0.05–0.1% Tween-20 for washes in immunoassays (WB, ELISA, IHC). Exact buffer compatibility for this item is not specified; consult CoA/Spec Sheet and your assay SOP.
• Blocking/dilution matrices: 1–5% BSA, nonfat dry milk, or serum can reduce nonspecific binding in many immunoassays. Compatibility with this antibody is not specified; optimize empirically.
• Avoid organic solvents (acetonitrile, methanol, DMSO) unless specifically recommended, as they may denature proteins.

Recommendation

• Focus on Application Protocols and Storage & Reconstitution for handling guidance. For sensitive applications (e.g., live-cell staining), verify preservative content and buffer composition on the CoA.

Storage & Reconstitution

Item-specific storage and shipping

• Storage conditions: Store at -20°C. Avoid repeated freezing and thawing.
• Shipping: Shipped in an ice chest with ice pads.
• Appearance, formulation, concentration, and reconstitution method: Not specified for this item; refer to CoA/Spec Sheet.

General best practices for antibodies (literature; not item-specific specifications)

• Aliquoting: Upon first thaw, divide into single-use aliquots sized for one experiment to prevent freeze–thaw–induced aggregation.
• Short-term storage: Many antibodies tolerate 2–8°C for up to 1–2 weeks once thawed; verify stability for this SKU on the CoA before adopting.
• Long-term storage: -20°C or -80°C per supplier guidance; avoid frost-free freezers and temperature cycling.
• Light and agitation: Protect from strong light; avoid vigorous vortexing and foaming.
• Thawing: Thaw on ice or at 2–8°C; mix gently by inversion.
• Reconstitution: If supplied lyophilized (not specified here), reconstitute with sterile water or buffer as recommended on the label/CoA, allowing the vial to equilibrate to RT before opening to prevent condensation ingress.

Documentation

• Before first use, record lot number, concentration, and aliquot map. Consult the CoA/SDS for any preservative content (e.g., sodium azide) and buffer composition that may impact downstream applications.

Structure & Identity

This product is a recombinant research antibody directed against the Cdc23 antigen.

• Item-specific identifiers
  • SKU: Ab095583
  • Product name: Recombinant Cdc23 Antibody
  • Category: Antibody (research use only)
• Small-molecule identifiers (CAS, CID, InChIKey, SMILES): Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General/literature description (antibody structure):

• Typical recombinant research antibodies are IgG-class immunoglobulins comprising two identical heavy chains and two identical light chains forming a Y-shaped heterotetramer stabilized by disulfide bonds.
• Domains: Variable regions (VH/VL) confer antigen binding; constant regions (CH/CL) define isotype and effector properties. The antigen-binding fragment (Fab) engages epitopes; the Fc mediates secondary interactions.
• 2D conceptual features: two Fab arms (paratopes) at the tips of the Y, connected via a hinge to an Fc stem. Glycosylation sites often occur in the Fc CH2 domain (literature).
• Typical intact IgG MW is ~150 kDa; fragments (Fab, scFv) are smaller (literature). The exact isotype/format for this item is not specified; refer to CoA/Spec Sheet.

Note: As a protein biologic, conventional small-molecule identifiers (SMILES/InChI) do not apply. Where exact chain sequences, isotype, or clonality are required (e.g., epitope mapping, sequence features), please consult the certificate of analysis (CoA) for this lot.

Synthetic Utility

Not typically applicable. This product is a recombinant antibody and is not used as a reagent for organic synthesis or as a building block.

For users seeking functionalization or conjugation (general guidance)

• Antibody conjugation to enzymes, fluorophores, or biotin can be achieved via amine-reactive (NHS-ester), thiol-reactive (maleimide), or click chemistry handles, provided the formulation is compatible (e.g., carrier-free, amine-free buffers).
• If conjugation is desired, verify the supplied buffer/excipients on the CoA; desalting into conjugation-friendly buffers (e.g., PBS without amines) may be required. This SKU’s formulation is not specified.

Otherwise, refer to Reaction & Applications and Application Protocols for immunoassay-related use cases.

Target Specificity

Item-specific information

• Target antigen: Cdc23 (as named in the product title).
• Additional specificity details (species reactivity, epitope, clone ID, isotype, host, cross-reactivity): Not specified for this item; refer to CoA/Spec Sheet.

Notes

• Without clone/isotype and epitope information, users should validate specificity in their system using appropriate controls: knockout/knockdown lysates, peptide/blocking controls if available, and orthogonal detection where possible.

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Cdc23 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.