PCR-ELISA Telomerase Assay
Telomeres are specific DNA-protein structures at the ends of eukaryotic chromosomes, and telomeric DNA is a series of repetitive G-rich DNA sequences, which are
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Construction of siRNA expression vector targeting EGFR gene
The constructed psj-EGFR vector was transfected into A549 cells using Lipofeetamine2000, and real-time quantitative fluorescence PCR, immunofluorescence and flo
Basic techniques for the preparation and use of PPA
Cross-linking of the initially purified SPA with HRP by sodium periodate oxidation (cross-linking reaction time at 22°C for 5h) gives PPA.
Transcription cDNA probe
A probe that has obtained mRNA from the transcription of the corresponding gene, which is then obtained by reverse transcription, is called a cDNa probe (cDNa p
X-ray crystallography for protein structure determination
X-ray diffraction is an extremely important method for determining the crystal structure of proteins. Biomolecule X-ray crystallography is the science of reveal
Protein transfection induced by ips cells
Heterodimeric expression of Oct4, Klf4, Sox2, and c-Myc transcription factors enables reprogramming of mouse somatic cells to induce functional stem cells. (1)
G protein-coupled receptor pathway: GC-cGMP-PKG
Extracellular signals are received by receptors and delivered to AC via Gs or Gi to activate or inhibit it; AC is activated to produce cAMP, which activates PKA
Methylation PCR
Methylation-Specific PCR (MS-PCR) is a new method developed by Herman et al. (1996) based on the use of bisulfite treatment. This method is highly sensitive and
Measurement of Ph in polluted water
Food factories require that the pH value of wastewater discharged be between 6.5 and 8.5. In the production process of fruit and vegetable sweat drink factories