Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.

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Almacenamiento y envío

Store at -20°C,Avoid repeated freezing and thawing Envío Ice chest + Ice pads. Consulte el COA específico del lote para conocer las especificaciones exactas.

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Documentos de calidad

SDS, COA, ficha técnica y hoja de especificaciones disponibles para descargar. COA específico del lote accesible mediante búsqueda por número de lote.

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Prueba documental

Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.

Descripción general

Mouse anti Human HDAC1 Antibody, Monoclonal (E22), could be used for WB, IHC, IF, ICC and so on.

Application:

WB: 0.2-2μg/mL

IHC: 5-20μg/mL

ICC/IF: 5-20μg/mL

Protein Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Post-translational

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.

Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.

Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Especificaciones

Product Name

HDAC1 Mouse mAb - Anticuerpo primario, specific to HDAC1, Mouse IgG1

Sinónimos

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Especies hospedadoras

Ratón

Especificidad

HDAC1

Inmunógeno

Recombinant HDAC1 expressed in E.coli (AA 1-482) .

Control positivo

WB: A549, HeLa, HepG2, Jurkat, THP-1, HEK293 and NIH/3T3 cell lysates; IHC: Human Placenta Tissue, Human Lung cancer Tissue, Rat Colon Tissue, Rat Testis Tissue, Mouse Pancreas Tissue, Mouse Cerebellum Tissue; IF: HeLa.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A+G Affinity Chromatography

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: HDAC1 Mouse mAb (Ab107009)

HDAC1 Mouse mAb (Ab107009) - Western Blot
All lanes: HDAC1 Mouse mAb (Ab107009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 63 kDa
Exposure time: 1.2 s