Trypsin, CAS No.9002-07-7

CAS: 9002-07-7 Cat. No.: T274333 Número EC: 607-831-3

CALIDAD & PUREZA EnzymoPure™, 1:250,Tissue Culture Grade

Accession #

P00761

Bioactivity

Trypsin>250 USP U/mg, Chymotrypsin <75 USP U/mg

Descripción general

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Trypsin Powder, Porcine 1:250 is used to release adherent cells from tissue culture plates for passaging. It was used in the isolation and culture of human endothelial cells.

Especificaciones

Product Name

Trypsin, CAS No.9002-07-7

Grado

EnzymoPure™

Especificaciones y pureza

EnzymoPure™, 1:250,Tissue Culture Grade

Mecanismos bioquímicos y fisiológicos

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxy

Bioactividad

Trypsin>250 USP U/mg, Chymotrypsin <75 USP U/mg

Número de adhesión

P00761

CAS

9002-07-7

Número de la Comisión de Enzimas

3.4.21.4

Tipo de molécula

Enzima

Almacenamiento y envío

Concentración

1:250,Tissue Culture Grade

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Definición de unidad

25℃， pH7.6， The reaction system is 3.0ml (1cm optical path), and the enzymatic hydrolysis of BAEE increases the absorption value at 253nm by 0.003 per minute, defined as one USP unit.