ABCB5 Mouse mAb - Anticorps primaire, specific to ABCB5, Mouse IgG1

Cat. No.: Ab086955

GRADE & PURITY Carrier Free, ExactAb™, Validated, See COA

Application

IHC, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG1

Clone Number

CHS11A2

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human ABCB5 Antibody, Monoclonal (CHS11A2), could be used for WB, IHC and so on.

Specifications

Product Name

ABCB5 Mouse mAb - Anticorps primaire, specific to ABCB5, Mouse IgG1

Synonymes

ABCB 5 antibody | ABCB5 antibody | ABCB5 P-gp antibody | ABCB5_HUMAN antibody | ABCB5alpha antibody | ABCB5beta antibody | ATP binding cassette protein antibody | ATP binding cassette sub family B (MDR/TAP) member 5 antibody | ATP binding cassette sub fam

Spécifications et pureté

Carrier Free, ExactAb™, Validated, See COA

Mécanismes biochimiques et physiologiques

Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.

Espèce hôte

Souris

Spécificité

ABCB5

Immunogène

KLH-conjugated synthetic peptide encompassing a sequence of human ABCB5.

Contrôle positif

WB: HeLa, HEK293 and Jurkat cell lysates. IHC: Human breast cancer tissue.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG1

SDS-PAGE

150 kDa

Méthode de purification

Antigen affinity purified

Concentration

See COA

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: ABCB5 Mouse mAb (Ab086955)

ABCB5 Mouse mAb (Ab086955) - Western Blot
All lanes: ABCB5 Mouse mAb (Ab086955) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 86 kDa
Exposure time: 100.0 s

IHC: ABCB5 Mouse mAb (Ab086955)

ABCB5 Mouse mAb (Ab086955) - IHC
Immunohistochemical analysis of ABCB5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ABCB5 Mouse mAb (Ab086955) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application

Application

Dilution info

WB

1/1000 - 1/2000

IHC

1/200-1/500

Cibles associées (humaines)

ABCB5 Tbio ATP-binding cassette sub-family B member 5 (0 Activities)

Discover Target: ABCB5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (ABCB5 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.