Rabbit Anti-Mouse IgG (Biotin) - Secondary antibody

Cat. No.: Ab176439
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. 1.0 mg/mL
Application
ELISA, WB
Host species
Rabbit
Reactive species
Mouse
Conjugation
HRP
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100μg
Ab176439-100μg
≥10
$28.90
1mg
Ab176439-1mg
1
$239.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing Ships Wet ice Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application
WB: 1:10,000-1:200,000
ELISA: 1:10,000-1:200,000

Specifications

Product Name
Rabbit Anti-Mouse IgG (Biotin) - Secondary antibody
Synonyms
Rabbit Anti-Mouse IgG/Biotin | Rabbit Anti-Mouse IgG-Biotin | Anti-Mouse IgG -Biotin antibody produced in rabbit
Specifications & Purity
ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL
Host species
Rabbit
Conjugation
HRP
Grade
Azide Free, ExactAb™, High Performance, Validated
Purification Method
Antigen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In
Wet ice
Stability And Storage
Store at 4℃. Avoid freeze/thaw cycle. Store in the dark.
Images

Rabbit Anti-Mouse IgG (Biotin) (Ab176439) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Rabbit Anti-Mouse IgG (Biotin) (Ab176439) at 1/40000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 8.4 s

Rabbit Anti-Mouse IgG (Biotin) (Ab176439) - ELISA
Rabbit Anti-Mouse IgG (Biotin) (Ab176439) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Rabbit Anti-Mouse IgG (Biotin) (Ab176439) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Application
ApplicationDilution info
WB

1/10000-1/200000

ELISA

1:10000-1:200000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202219Certificate of AnalysisApr 13, 2026 Ab176439
ZJ23F1202220Certificate of AnalysisApr 13, 2026 Ab176439
ZJ23R1100137Certificate of AnalysisNov 15, 2023 Ab176439
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →

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