GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Sans transporteur, ExactAb™, validé, 1,0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conserver à -80°C, éviter les congélations et décongélations répétées Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Vue d’ensemble
Anticorps monoclonal de souris anti-SCAMP5 humain (K06322_12G11), pouvant être utilisé pour WB, IHC, IP, etc. Application : WB : 1/1000 IHC : 1/200 IP : 1/100 Fonction de la protéine Requis pour l'exocytose dépendante du calcium des cytokines contenant une séquence de signal telles que CCL5. Agit probablement en coopération avec la machinerie SNARE. Peut jouer un rôle dans l'accumulation de la protéine polyglutamine (polyQ) élargie huntingtine (HTT) en cas de stress du réticulum endoplasmique en inhibant la voie d'endocytose
Specifications
Product Name
AcM de souris SCAMP5 - Anticorps primaire, specific to SCAMP5, Mouse IgG1
Conserver à -80°C, éviter les congélations et décongélations répétées
Expédié en
Dry ice packs + Cold packs
Stabilité et stockage
Stocker à 2-8 ℃ (3 mois). Stocker à -20 ℃ (12 mois). Stocker à -80 ℃ (36 mois). Dès réception, il est recommandé d'aliquoter. Éviter les cycles de congélation/décongélation.
Images
SCAMP5 Mouse mAb (Ab126689) - Western Blot All lanes: SCAMP5 Mouse mAb (Ab126689) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 26 kDa Observed band size: 24 kDa Exposure time: 100.0 s
SCAMP5 Mouse mAb (Ab126689) - IHC IHC analysis of cerebellum tissue by SCAMP5 Mouse mAb (Ab126689). IHC was performed using sections of the formalin-fixed paraffin-embedded cerebellum tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SCAMP5 Mouse mAb (Ab126689) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Cytoplasm of cells in granular layer and in molecular layer are positively stained.
SCAMP5 Mouse mAb (Ab126689) - IP Immunoprecipitation was performed by incubation of 2.5 µg of SCAMP5 Mouse mAb (Ab126689) with mouse brain lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. SCAMP5 Mouse mAb (Ab126689) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: Mouse brain lysate Lane 2: SCAMP5 immunoprecipitated from mouse brain lysate by SCAMP5 Mouse mAb (Ab126689) Lane 3: The same as Lane 2 but Mouse IgG was used as IgG isotype control antibody Result: SCAMP5 Mouse mAb (Ab126689) can immunoprecipitate SCAMP5.
Application
Application
Dilution info
WB
1/1000
IP
1/100
IHC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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