AIMP3/p18 Mouse mAb - Anticorps primaire, specific to EEF1E1, Mouse IgG2b

Cat. No.: Ab087803

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Souris

Reactive species

Humain, Souris

Isotype

Mouse IgG2b

Clone Number

E94S639

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human AIMP3/p18 Antibody, Monoclonal (E94S639), could be used for WB, IF/ICC and so on.

Specifications

Product Name

AIMP3/p18 Mouse mAb - Anticorps primaire, specific to EEF1E1, Mouse IgG2b

Synonymes

AIMP 3 | AIMP3 | Aminoacyl tRNA synthetase complex interacting multifunctional protein 3 | Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 | ARS interacting multifunctional protein 3 | Cyclin dependent kinase 6 inhibitor | Elongati

Spécifications et pureté

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Mécanismes biochimiques et physiologiques

Positive modulator of ATM response to DNA damage.

Espèce hôte

Souris

Spécificité

EEF1E1

Immunogène

Recombinant fusion protein of human EEF1E1.

Contrôle positif

WB: Mouse testis, Rat brain, Hep G2, A549, A431, Jurkat and K562 lysates. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG2b

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

0.5 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: AIMP3/p18 Mouse mAb (Ab087803)

AIMP3/p18 Mouse mAb (Ab087803) - Western Blot
All lanes: AIMP3/p18 Mouse mAb (Ab087803) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 12.0 s

Western Blot: AIMP3/p18 Mouse mAb (Ab087803)

AIMP3/p18 Mouse mAb (Ab087803) - Western Blot
All lanes: AIMP3/p18 Mouse mAb (Ab087803) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 25.5 s

IF/ICC: AIMP3/p18 Mouse mAb (Ab087803)

AIMP3/p18 Mouse mAb (Ab087803) - IF/ICC
Immunofluorescent analysis of EEF1E1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with AIMP3/p18 Mouse mAb (Ab087803) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Application

• Application: Dilution info
• ICC/IF:

  1/10-1/50 ;

• WB:

  1/500-1/2000 ;

Cibles associées (humaines)

EEF1E1 Tbio Eukaryotic translation elongation factor 1 epsilon-1 (0 Activities)

Discover Target: EEF1E1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (AIMP3/p18 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.