Avidin from egg white, CAS No.1405-69-2

CAS: 1405-69-2 Cat. No.: A103033 Numéro CE: 215-783-6

QUALITÉ & PURETÉ Bioactive, Native, ≥98%(SDS-PAGE), ≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Accession #

P02701

Bioactivity

≥10 U/mg solid; >10 U/mg protein

Vue d’ensemble

Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.

Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including Western Blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.

Avidin and biotin are routinely conjugated to indicator enzymes, such as alkaline phosphatase and horseradish peroxidase for use in diagnostic kit development and manufacturing.

Application：

Avidin from egg white can be used in assays for functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Spécifications

Product Name

Avidin from egg white, CAS No.1405-69-2

Synonymes

Avidin from egg white | Avidin, chiken egg white | Avidin, Egg White | Anti-biotin protein | Immunopure Avidin | AVD

Grade

Bioactive, Native

Spécifications et pureté

Bioactive, Native, ≥98%(SDS-PAGE), ≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Mécanismes biochimiques et physiologiques

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin). Post-translational: N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)

Bioactivité

≥10 U/mg solid; >10 U/mg protein

Accession #

P02701

Poids moléculaire prévu

64,000 (tetramer of 16,000 MW subunits)

CAS

1405-69-2

Type de molécule

Protein

Product Line

Produits biologiques

Stockage et expédition

Concentration

≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Reconstitution

Reconstitute in 0.1 M phosphate buffer, pH 6.8.

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at -20℃ long term (36 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Définition de l'unité

One unit will bind one microgram of d-biotin.