beta 2 Microglobulin Mouse mAb - Anticorps primaire, specific to beta 2 Microglobulin, IgG2a

Cat. No.: Ab169199

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 1.0 mg/mL

Application

IF/ICC, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG2a

Clone Number

CD01/2B7

Conjugation

Unconjugated

Vue d’ensemble

Application
WB: 1/1,000 - 1/10,000; Predicted molecular weight: 13.7 KDa
ICC/IF: 1/200

Spécifications

Product Name

beta 2 Microglobulin Mouse mAb - Anticorps primaire, specific to beta 2 Microglobulin, IgG2a

Synonymes

B2M | B2MG_HUMAN | Beta 2 microglobin | Beta 2 microglobulin | Beta 2 microglobulin precursor | Beta chain of mhc class 1 proteins | Beta chain of MHC class I molecules | Beta-2-microglobulin form pI 5.3 | CDABP0092 | Hdcma22p | IMD43 | beta-2-microglobul

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL

Espèce hôte

Souris

Spécificité

beta 2 Microglobulin

Immunogène

Recombinant protein corresponding to Human beta-2-microglobulin

Contrôle positif

WB: HeLa, Raji, THP-1 and HepG2 Whole cell Lysates ICC/IF: HeLa cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotype

IgG2a

Type de chaîne lumineuse

kappa

SDS-PAGE

150.0 KDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Western Blot: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

Western Blot: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

Western Blot: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

Western Blot: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

ICC/IF: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - ICC/IF
IF analysis of beta 2 Microglobulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta 2 Microglobulin Mouse mAb (Ab169199) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application

Dilution info

IF/ICC

1/200

WB

1/1,000 - 1/10,000; Predicted molecular weight: 13.7 KDa

Cibles associées (humaines)

B2M Tbio Beta-2-microglobulin (0 Activities)

Discover Target: B2M

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (beta 2 Microglobulin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.