CCL15 Mouse mAb: Specificity, Applications & Key Facts

CCL15 Mouse mAb is a monoclonal antibody raised in mouse and designed to specifically detect human CCL15, a CC-family chemokine implicated in immune cell recruitment and inflammatory signaling.

Product SKU

Ab350899

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target

CCL15 (CC Chemokine Ligand 15)

Formulation

PBS (phosphate-buffered saline)

Reference Clone

absea#KC-1446

Host Species

Mouse

Target Biology: What Is CCL15?

CCL15, also known as CC chemokine ligand 15 (previously designated MIP-5 or HCC-2), is a member of the CC chemokine superfamily. Chemokines of this class are characterized by two adjacent N-terminal cysteine residues and play pivotal roles in directing leukocyte migration to sites of inflammation or injury.

CCL15 signals primarily through the chemokine receptors CCR1 and CCR3, which are expressed on monocytes, neutrophils, eosinophils, and dendritic cell subsets. Through receptor engagement, CCL15 promotes the chemotaxis of these immune effector cells, contributing to both innate and adaptive immune responses.

Dysregulated CCL15 expression has been reported in contexts including colorectal cancer, inflammatory bowel disease, and other inflammatory conditions, making it a relevant target for translational immunology research.

Antibody Characteristics & Formulation

CCL15 Mouse mAb (Ab350899) is produced by immunizing mice with CCL15 antigen, followed by hybridoma generation and rigorous clone selection to ensure monospecificity. The monoclonal format confers high lot-to-lot consistency and reproducibility compared with polyclonal preparations, critical for quantitative and comparative experiments.

The antibody is supplied in phosphate-buffered saline (PBS) without additives such as BSA or azide, which provides flexibility for downstream conjugation workflows or direct use in cell-based assays where carrier proteins or preservatives may interfere.

The internal reference designation absea#KC-1446 is associated with the hybridoma clone identity and may be used for cross-referencing with manufacturer quality-control records.

Recommended Applications

Monoclonal antibodies targeting CCL15 are typically applied across several immunological detection and functional platforms. Researchers should validate performance in each specific application and sample type:

• ELISA (Enzyme-Linked Immunosorbent Assay): Quantitative detection of soluble CCL15 in serum, plasma, cell culture supernatants, or tissue lysates.
• Western Blot (WB): Detection of CCL15 protein by molecular weight under denaturing or native conditions in cell or tissue extracts.
• Immunohistochemistry (IHC): Localization of CCL15 expression in formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections.
• Immunofluorescence (IF): Visualization of CCL15 in fixed cells or tissue sections using fluorophore-conjugated secondary antibodies.
• Flow Cytometry: Detection of intracellular or surface CCL15 on single-cell suspensions.

Note: Specific validated applications for Ab350899 should be confirmed with the Aladdin Scientific technical team, as application suitability depends on epitope accessibility and optimization conditions.

Storage, Handling & Quality Considerations

To maintain antibody integrity and ensure experimental reproducibility, observe the following best practices:

• Storage Temperature: Store at –20 °C for long-term archival. Aliquot upon receipt to avoid repeated freeze-thaw cycles, which can reduce binding activity.
• Working Concentration: Titrate the antibody empirically for each application and detection system; optimal concentrations vary by assay format and sample matrix.
• Formulation Compatibility: The PBS-only formulation is compatible with most conjugation chemistries (e.g., NHS-ester labeling) and does not introduce azide interference in cell viability assays.
• Lot Consistency: As a monoclonal antibody, Ab350899 provides consistent epitope recognition across production lots. Retain lot-specific datasheets for regulatory or publication records.

Always consult the most current product datasheet available on the Aladdin Scientific website for lot-specific quality-control data and verified storage conditions.

Frequently asked questions

What is the host species and antibody format of Ab350899?

Ab350899 is a mouse monoclonal antibody (mAb) raised against CCL15. The monoclonal format ensures high specificity and lot-to-lot reproducibility compared with polyclonal alternatives.

What buffer or formulation is CCL15 Mouse mAb supplied in?

The antibody is supplied in PBS (phosphate-buffered saline) only, without BSA or azide preservatives. This carrier-free formulation is suitable for direct conjugation and for assays sensitive to azide or exogenous proteins.

Which chemokine receptors does CCL15 signal through, and why is this relevant for my research?

CCL15 primarily signals through CCR1 and CCR3, receptors expressed on monocytes, neutrophils, eosinophils, and dendritic cells. Researchers studying inflammation, cancer microenvironment, or allergic disease may find CCL15 particularly relevant as a mediator of leukocyte trafficking.

How should I store CCL15 Mouse mAb to preserve its activity?

Store the antibody at –20 °C for long-term use. Upon receipt, prepare single-use aliquots to avoid repeated freeze-thaw cycles. Refer to the product datasheet for the most current lot-specific recommendations.

Can I use Ab350899 for applications other than ELISA?

Monoclonal anti-CCL15 antibodies are broadly used in Western blot, immunohistochemistry, immunofluorescence, and flow cytometry. However, suitability of Ab350899 for specific applications must be validated empirically. Contact Aladdin Scientific's technical support team for application-specific guidance.

References

1. Pardigol A, et al. HCC-2, a human chemokine: gene structure, expression pattern, and biological activity. Proc Natl Acad Sci USA. 1998.
2. Zlotnik A, Yoshie O. Chemokines: a new classification system and their role in immunity. Immunity. 2000;12(2):121-127.
3. Rot A, von Andrian UH. Chemokines in innate and adaptive host defense: basic chemokinese grammar for immune cells. Annu Rev Immunol. 2004;22:891-928.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.