Experiments on the effect of hemolysis and jaundice on the determination of enzyme activity

Summary

To examine the accuracy of enzyme activity measurement in the presence of interfering substances, this experiment is from the Mudanjiang Medical College undergraduate 5-year laboratory guide for laboratory tests.

Operation method

Experiments on the effect of hemolysis and jaundice on the determination of enzyme activity

Principle

L-alanine + α-ketoglutarate ALT pyruvate + L-glutamate pyruvate + NADH + H+ → L-lactate + NAD+

Materials and Instruments

Jaundice Serum
ALT/GPT reagent
Semi-automatic biochemical analyzer Test tubes Sampler

Move

1. Dilute the reagent.

2、Pre-temperature up to 37 degrees.

3、Select the measurement program．

4, test:

5. Analysis of results:

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