PDLIM1 Mouse mAb: Key FAQs for Research Use

PDLIM1 Mouse mAb is a monoclonal antibody raised in mouse targeting the PDLIM1 protein, a PDZ and LIM domain-containing scaffolding protein implicated in cytoskeletal organization and signal transduction.

Product SKU

Ab350620

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

PDLIM1 (PDZ and LIM domain protein 1)

Formulation

PBS only

Supplier Reference

absea KC-51297

Species Reactivity

To be confirmed; please contact Aladdin Scientific for species reactivity details

What Is PDLIM1 and Why Is It a Research Target?

PDLIM1, also known as CLP-36 or elfin, is a member of the PDZ and LIM domain protein family. It contains an N-terminal PDZ domain and a C-terminal LIM domain, which together allow it to function as a molecular scaffold bridging cytoskeletal elements and signaling proteins. PDLIM1 has been studied in the context of actin cytoskeleton regulation, stress fiber formation, and various signaling cascades relevant to cell migration and differentiation. Its expression profile across tissues and its potential roles in oncogenesis make it a target of interest in both basic cell biology and translational cancer research.

Product Specifications and Formulation Details

The PDLIM1 Mouse mAb (Ab350620) is a monoclonal antibody of mouse origin supplied by Aladdin Scientific with the manufacturer reference absea KC-51297. The antibody is formulated in PBS (phosphate-buffered saline) only, without preservatives such as sodium azide or carrier proteins such as BSA unless otherwise stated in the specific lot documentation. Researchers should verify the exact concentration, lot-specific titer, and any additives by consulting the Certificate of Analysis (CoA) provided with each shipment.

Storage conditions and expiry information should be confirmed from the product datasheet and CoA. Typically, antibodies formulated in PBS without preservatives are recommended for storage at -20°C with avoidance of repeated freeze-thaw cycles, but users must follow the lot-specific instructions supplied with Ab350620.

Recommended Applications and Usage Guidelines

Monoclonal antibodies targeting PDLIM1 are commonly evaluated for use in applications including Western blotting (WB), immunofluorescence (IF), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). However, the validated applications for Ab350620 specifically should be confirmed by consulting the product datasheet or contacting Aladdin Scientific technical support, as not all formats are validated for every antibody lot.

When using this antibody, researchers should optimize primary antibody dilutions empirically for their specific experimental system, cell or tissue type, and detection platform. Appropriate positive and negative controls are strongly recommended to ensure specificity of the signal observed.

Ordering, Storage, and Handling

PDLIM1 Mouse mAb (Ab350620) can be ordered directly through Aladdin Scientific's online platform or by contacting the Aladdin Scientific sales team. When placing an order, please quote the SKU Ab350620 to ensure accurate fulfillment. Bulk quantities, custom formats, or special packaging requirements can be discussed with the Aladdin Scientific team.

Upon receipt, inspect the shipment for any signs of damage or temperature excursion. Store the antibody according to the instructions on the vial label and CoA. As a general guideline for PBS-only formulations, aliquoting into single-use volumes before freezing is advisable to minimize freeze-thaw degradation. Keep detailed records of lot numbers and storage conditions for reproducibility and regulatory compliance purposes.

Technical Support and Quality Assurance

Aladdin Scientific is committed to providing high-quality research reagents and reliable technical support. If you encounter issues with Ab350620 — such as no signal, high background, or unexpected bands — please contact Aladdin Scientific technical support with your experimental details, including the sample type, protocol, and detection system used. The technical team can assist with troubleshooting and, where applicable, facilitate returns or replacements in accordance with Aladdin Scientific's returns and refunds policy.

Every lot of Ab350620 is quality-controlled by the manufacturer absea under reference KC-51297. Lot-specific quality control data, including SDS-PAGE purity data and functional test results, are available upon request via the Certificate of Analysis.

Frequently asked questions

What species does PDLIM1 Mouse mAb (Ab350620) react with?

The species reactivity of Ab350620 is not explicitly stated in the current source material. Please consult the product datasheet available from Aladdin Scientific or contact technical support at Aladdin Scientific for confirmed species reactivity information before designing your experiment.

What buffer is PDLIM1 Mouse mAb (Ab350620) supplied in, and does it contain preservatives?

Ab350620 is formulated in PBS (phosphate-buffered saline) only. Based on the available source information, no additional preservatives or carrier proteins are indicated; however, researchers should verify this by reviewing the Certificate of Analysis provided with their specific lot, as formulation details can vary between lots.

What applications has PDLIM1 Mouse mAb (Ab350620) been validated for?

The source material does not specify validated applications for Ab350620. Typical applications for PDLIM1 monoclonal antibodies include Western blotting, immunofluorescence, and immunoprecipitation, but users must confirm validated applications for this specific product by consulting the official Aladdin Scientific product datasheet or contacting technical support.

How should PDLIM1 Mouse mAb (Ab350620) be stored to maintain activity?

While general best practice for PBS-formulated monoclonal antibodies without preservatives recommends storage at -20°C in single-use aliquots to avoid repeated freeze-thaw cycles, the precise storage conditions for Ab350620 should be confirmed from the vial label and the lot-specific Certificate of Analysis supplied by Aladdin Scientific.

How do I place an order for PDLIM1 Mouse mAb (Ab350620) from Aladdin Scientific?

You can order Ab350620 directly through Aladdin Scientific's online store by searching for the SKU Ab350620, or by contacting the Aladdin Scientific sales team directly. Please quote the SKU Ab350620 when placing your order to ensure accurate processing. For bulk orders or special requirements, contact Aladdin Scientific's sales team for assistance.

References

1. Aladdin Scientific product listing for PDLIM1 Mouse mAb, SKU Ab350620; manufacturer reference absea KC-51297.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.