PLXDC2 Mouse mAb: Applications, Biology & Protocols
PLXDC2 Mouse mAb: Applications, Biology & Protocols
PLXDC2 Mouse mAb is a monoclonal antibody targeting the PLXDC2 protein, a plexin domain-containing transmembrane receptor implicated in angiogenesis and tumor vasculature biology.
- Catalog Number
- Ab350636
- Antibody Type
- Mouse Monoclonal Antibody (mAb)
- Formulation
- PBS only
- Target Protein
- PLXDC2 (Plexin Domain-Containing Protein 2, also known as TEM7L)
- Host Species
- Mouse
- Storage Recommendation
- Store at 4°C for short-term; aliquot and store at -20°C or -80°C for long-term use. Avoid repeated freeze-thaw cycles.
Target Biology: What Is PLXDC2?
PLXDC2 (Plexin Domain-Containing Protein 2), also referred to as TEM7L (Tumor Endothelial Marker 7-Like), is a single-pass transmembrane receptor belonging to the plexin domain-containing protein family. It is expressed predominantly on endothelial cells and has been identified as a marker of tumor vasculature, making it of significant interest in cancer biology and angiogenesis research.
Structurally, PLXDC2 contains an extracellular plexin domain that is thought to mediate ligand binding and cell–cell or cell–matrix interactions. Studies suggest that PLXDC2 plays a role in regulating endothelial cell behavior, including proliferation and migration, processes central to neovascularization. Its selective expression in tumor-associated blood vessels positions it as a potential biomarker and therapeutic target in oncology.
Research into PLXDC family members has also indicated roles in neural development and stem cell biology, broadening the relevance of PLXDC2-targeting tools across multiple fields of investigation.
Product Description & Formulation
The PLXDC2 Mouse mAb (Catalog No. Ab350636) is a mouse-derived monoclonal antibody generated against the PLXDC2 antigen. Monoclonal antibodies offer the advantage of exceptional lot-to-lot consistency, high specificity for a single epitope, and reproducible performance across experiments—critical attributes for publication-quality data.
This antibody is supplied in PBS (Phosphate-Buffered Saline) only, without carrier proteins such as BSA or preservatives such as sodium azide. A PBS-only formulation is particularly advantageous for downstream applications requiring conjugation (e.g., fluorescent dye labeling, biotin conjugation) or for use in functional assays where exogenous proteins could confound results.
Researchers should note that the absence of stabilizing carrier proteins may necessitate careful handling. It is recommended to aliquot the antibody upon receipt to prevent degradation from repeated freeze-thaw cycles, and to add a carrier protein such as BSA (0.1–1%) if long-term stability in dilute solutions is required.
Recommended Applications & Usage Guidelines
While specific validated application data should be confirmed from the official product datasheet, monoclonal antibodies of this class are commonly employed in the following research applications:
- Western Blotting (WB): Detection of PLXDC2 protein expression in cell lysates or tissue extracts under denaturing conditions.
- Immunofluorescence (IF) / Immunocytochemistry (ICC): Visualization of PLXDC2 subcellular localization in fixed cells.
- Immunohistochemistry (IHC): Assessment of PLXDC2 expression patterns in formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections, particularly tumor and vascular tissues.
- Flow Cytometry (FC): Quantification of surface PLXDC2 expression on intact cells, useful for endothelial cell profiling.
- Enzyme-Linked Immunosorbent Assay (ELISA): Quantitative detection of PLXDC2 in solution-phase formats.
Optimal working dilutions and antigen retrieval conditions vary by application and sample type. Users are strongly encouraged to perform titration experiments to determine the optimal antibody concentration for their specific experimental system. Always include appropriate positive and negative controls.
Storage, Handling & Stability
Proper storage and handling of the PLXDC2 Mouse mAb are essential to maintaining its activity and specificity over time. The following guidelines represent general best practices for monoclonal antibodies supplied in PBS without preservative:
- Short-term storage (up to 1 month): Store at 2–8°C (4°C refrigerator). Keep away from light.
- Long-term storage: Aliquot into single-use volumes upon receipt and store at -20°C or -80°C. Aliquoting prevents activity loss from repeated freeze-thaw cycles.
- Freeze-thaw cycles: Limit to a maximum of 2–3 cycles. Each freeze-thaw event can cause protein aggregation and reduce specific activity.
- Avoid: Storing at room temperature for extended periods, exposure to direct sunlight, and repeated freeze-thaw cycles.
- Working solutions: Prepare fresh dilutions in an appropriate buffer (e.g., PBS with 1% BSA) prior to each experiment. Do not store working dilutions long-term.
Because the formulation is PBS only, no azide is present; therefore, standard biosafety precautions for protein reagents apply. Consult your institution's biosafety guidelines for handling recombinant proteins and antibody reagents.
Frequently asked questions
What is the formulation of PLXDC2 Mouse mAb (Ab350636)?
The PLXDC2 Mouse mAb (Ab350636) is supplied in PBS (Phosphate-Buffered Saline) only, without carrier proteins such as BSA or preservatives such as sodium azide. This makes it particularly suitable for conjugation workflows and functional assays where exogenous proteins may interfere.
How should I store the PLXDC2 Mouse mAb to maintain its activity?
For short-term use (up to one month), store the antibody at 2–8°C. For long-term storage, aliquot the antibody into single-use volumes upon receipt and store at -20°C or -80°C. Repeated freeze-thaw cycles should be minimized (no more than 2–3 cycles) to prevent protein aggregation and loss of activity.
What research applications is PLXDC2 Mouse mAb suitable for?
Monoclonal antibodies in this class are commonly used in Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC), and ELISA. However, users should consult the official product datasheet for validated applications specific to Ab350636, and perform titration experiments to optimize conditions for their system.
Why is a PBS-only formulation beneficial for my experiments?
A PBS-only formulation (without BSA or azide) ensures that the antibody is compatible with conjugation chemistries such as fluorescent dye or biotin labeling, since carrier proteins can compete in conjugation reactions. It also eliminates azide interference in cell-based or enzymatic assays. Researchers planning conjugation or functional studies particularly benefit from this formulation.
What is the biological significance of PLXDC2 in disease research?
PLXDC2 is a transmembrane receptor enriched in tumor-associated endothelial cells and has been studied as a tumor endothelial marker (TEM7L). Its preferential expression in tumor vasculature makes it a candidate biomarker for cancer diagnostics and a potential target for anti-angiogenic therapies. Research tools such as PLXDC2 Mouse mAb enable scientists to investigate its expression, localization, and function in both normal and pathological tissues.
References
- Bagley RG et al. Tumor endothelial markers (TEMs) as targets for anti-angiogenic therapy. Pharmacology & Therapeutics. Review of TEM family including TEM7L/PLXDC2.
- Nissen LJ et al. Angiogenic markers associated with tumor endothelial cells. General reference for PLXDC family biology and tumor vasculature markers.
- Aladdin Scientific. Product Datasheet: PLXDC2 Mouse mAb, Cat. No. Ab350636. Aladdin Scientific internal reference.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.