PON1/PON2 Mouse mAb: FAQs for Research Use

PON1/PON2 Mouse mAb is a mouse monoclonal antibody targeting the paraoxonase 1 and paraoxonase 2 proteins, supplied by Aladdin Scientific (SKU Ab350643) as a PBS-formulated reagent for research applications.

SKU

Ab350643

Clone / Source Reference

absea KC-3774

Host Species

Mouse

Antibody Format

Monoclonal antibody (mAb)

Formulation Buffer

PBS only

Target Proteins

PON1 and PON2 (Paraoxonase 1 and Paraoxonase 2)

What Are PON1 and PON2?

PON1 (Paraoxonase 1) and PON2 (Paraoxonase 2) are members of the paraoxonase gene family of calcium-dependent lactonases and esterases. PON1 is primarily found circulating in plasma, where it associates with high-density lipoprotein (HDL) and plays a role in the hydrolysis of oxidized lipids, contributing to antioxidant defense. PON2 is an intracellular enzyme expressed broadly across tissues. Both enzymes are studied in the context of cardiovascular disease, oxidative stress, and organophosphate detoxification. Researchers require reliable antibodies capable of detecting one or both paralogs to study their expression, localization, and function.

Product Specifications and Formulation

The PON1/PON2 Mouse mAb (Aladdin Scientific SKU: Ab350643) is a mouse-derived monoclonal antibody referenced under clone identifier KC-3774 from absea. The antibody is formulated exclusively in PBS (phosphate-buffered saline) with no additional stabilizers, carriers, or preservatives stated in the current product record. Researchers should note that a PBS-only formulation may require the addition of a carrier protein (e.g., 0.1–1% BSA) and a preservative (e.g., 0.02% sodium azide) for long-term storage stability, though specific guidance should be confirmed with Aladdin Scientific technical support before use.

Recommended Storage and Handling

As a general best practice for PBS-formulated monoclonal antibodies, short-term storage (up to 4 weeks) is typically at 2–8 °C, while long-term storage is recommended at −20 °C or −80 °C in single-use aliquots to avoid repeated freeze-thaw cycles. Repeated freeze-thaw cycles can lead to aggregation and loss of antibody activity. Because specific storage conditions for Ab350643 are not fully detailed in the current product record, users are strongly encouraged to contact Aladdin Scientific customer support or consult the product datasheet upon receipt for validated storage recommendations.

Ordering and Support

PON1/PON2 Mouse mAb can be ordered directly through the Aladdin Scientific website using SKU Ab350643. For bulk quantities, custom formulations, or additional technical specifications such as validated applications, species cross-reactivity, and lot-specific quality control data, please contact Aladdin Scientific's sales or technical support team. Procurement teams should reference SKU Ab350643 and clone KC-3774 when placing orders or requesting quotations to ensure the correct product is supplied.

Frequently asked questions

What proteins does the PON1/PON2 Mouse mAb (Ab350643) recognize?

The PON1/PON2 Mouse mAb is designed to recognize PON1 (Paraoxonase 1) and PON2 (Paraoxonase 2), two related members of the paraoxonase enzyme family. The exact epitope and cross-reactivity details should be confirmed with Aladdin Scientific technical support or by consulting the product datasheet, as these specifics are not fully described in the current product record.

What buffer is the PON1/PON2 Mouse mAb supplied in?

According to the product information, Ab350643 is formulated in PBS (phosphate-buffered saline) only, with no additional excipients listed. Researchers planning long-term storage should consider adding a carrier protein and preservative; please verify with Aladdin Scientific before modifying the formulation.

What applications is the PON1/PON2 Mouse mAb validated for?

The current product record does not specify validated applications for Ab350643. Common applications for mouse monoclonal antibodies targeting intracellular or plasma enzymes such as PON1 and PON2 include Western blotting, ELISA, immunofluorescence, and immunoprecipitation, but users must confirm validated applications with Aladdin Scientific or review the product datasheet before experimental use.

How should I store the PON1/PON2 Mouse mAb after receipt?

While the product datasheet should be consulted upon receipt for validated storage instructions, general best practice for PBS-formulated monoclonal antibodies is to store at 2–8 °C for short-term use and at −20 °C or −80 °C in single-use aliquots for long-term preservation. Avoid repeated freeze-thaw cycles to maintain antibody integrity.

What is the clone reference for Ab350643 and what does it mean?

The clone reference for Ab350643 is KC-3774, sourced from absea. This identifier traces the hybridoma clone from which the monoclonal antibody is derived, ensuring lot-to-lot consistency and specificity. When ordering or requesting technical documentation, referencing both the Aladdin SKU (Ab350643) and the clone identifier (KC-3774) helps ensure accurate product identification.

References

1. Product record: PON1/PON2 Mouse mAb, SKU Ab350643, Aladdin Scientific. Clone KC-3774 (absea). Formulation: PBS only.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.