3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Wild-type strains obtained from nature are generally able to grow on basic medium. After physical or chemical treatment, some of the wild-type cells have mutate
DNA probes are divided into two categories: isotope-labeled probes and non-isotope-labeled probes. Isotope-labeled probes usually have high radioactivity and hi
Genes are the smallest functional units of biological genetic material, and genes are separable. To determine the boundaries of a gene, it is not possible to re
Deletion localization is a gene localization method in which a strain at the mutation position to be tested is recombined separately with a series of mutant str
The distance between two genes can be measured by the number of recombinants in the progeny after two- and three-point crosses. The closer the distance between
Chloroplasts are unique organelles in plant cells and contain DNA. chloroplast DNA is in the form of a ring, and the chloroplast DNA (cpDNA) of higher plants va
In eukaryotes, in addition to the nuclear genome, there are cytoplasmic genomes, i.e., the mitochondrial genome and, in green plants, the chloroplast genome. Bo
Lambda phage is a commonly used biological material in molecular genetics research, and the enzymatically fragmented segments of its DNA are often used as stand
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