3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
The sugar fermentation test is the most commonly used biochemical reaction and is particularly important in the identification of intestinal bacteria. The vast
Protoplasts are formed by the differentiation of protoplasm, specifically the cell membrane and intramembranous cytoplasm and other life-active organelles. In p
Under non-selective growth conditions, most E. coli/yeast shuttle vectors are lost at a frequency of about 1%, and one plasmid that is not easily isolated and r
Because of the simplicity and sensitivity of the β-galactosidase assay, yeast genes are frequently labeled with the functional portion of the lacZ gene to indi
The nucleus (nucleus) is the largest and most important organelle in the cell (the old junior high school textbooks believe that the nucleus is not an organelle
Slides coated with polylysine (a Thermo product) with durable bioadhesion for attaching frozen and paraffin-embedded sections that can be subsequently centrifug
A general method for cloning yeast genes by complementation using a putative mutation. The mutation is cdc101-1, which is both recessive and temperature sensiti
The role of fixation, from the histological point of view its simple definition is to maintain the intrinsic morphology and structure of cells and tissues, so t
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