Influenza virus RNA extraction
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
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Purification experiments of U series small ribonucleoprotein particles
The splicosome is the catalytic unit for excising introns from primary transcripts in eukaryotes. The splicosome contains four small nuclear ribonucleoprotein p
SELEX screening of randomized RNA libraries
SELEX screening experiments of randomized RNA libraries can be used for (1) in vitro diagnosis; (2) in vivo therapy, etc.
Encephalitis B virus RNA extraction
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
Preparation of antisense RNA
RNA can also be synthesized in vitro by RNA polymerases (e.g., T3, T7, or SP6 ) using linear DNA as a template. This experiment is based on the "RNA Laboratory
miRNA cloning assay
In addition to the familiar mRNA, there are also a large number of non-coding RNA (ncRNA) that do not code for proteins. Non-coding RNA plays a very important r
Isolation of RNA from Gram-positive bacteria
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
SELEX screening of single-stranded DNA libraries
SELEX screening experiments of single-stranded DNA libraries can be used for (1) in vitro diagnostics; (2) in vivo therapeutics, etc.
Preparation of nuclear and cytoplasmic S100 extracts of HeLa cells for in vitro splicing reaction assay
Cytosolic extracts can be used for splicing of precursor mRNAs. The S100 extract, although it includes many splicing factors, does not have splicing activity be