Isolation of RNA based on size
RNA samples may be denatured by treatment with formamide and by electrophoretic separation on gels containing formaldehyde, a method modified from Lehrachetal.
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Southern blotting technology
Blot hybridization is the process of binding a nucleic acid fragment to be tested to a certain solid phase support and then hybridizing it to a labeled probe pr
quantitative PCR
Quantitative PCR can be used for: (1) quantification of the amount of PCR starting template by analyzing the PCR end-product or monitoring the PCR process using
Agarose gel electrophoresis experiment
Agarose gel electrophoresis can be applied to (1) DNA cutting and recovery, (2) DNA isolation, (3) corroboration of whether DNA is recombinant or not, whether p
Determination of DNA concentration and purity
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In-situ notch panning technique
The in situ nick translation technique is also known as in situ nick translation. It was first used to study DNA replication in vitro, and has since been extend
Microbial Genome DNA Extraction
The yeast is first lysed using a solution containing leaving salts to ensure complete denaturation of the macromolecules. Afterwards, ethanol is added and the D
Establishment of an insect baculovirus expression system
The baculoviruses are double-stranded DNA viruses that infect mainly insects, but also in vitro insect cell lines, and are not toxic to humans or animals. Alfal
Polyclonal antibody purification assay
The octanoic acid extraction method can be used to isolate and extract Ig from human, rabbit and mouse serum and McAb from hybridoma-induced ascites and culture