3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Learning the electrophoresis technique of separating proteins in the gel and the immunodiffusion technique of using the specific reaction of antigen and antibod
The basic techniques for preparing cell suspensions from mouse lymphoid organs are first described, including the removal of erythrocytes and dead cells
The met
This unit describes two methods of isolating dendritic cells (DCs): isolating DCs from mouse spleen and obtaining large numbers of DCs from mouse bone marrow pr
After mixing the sample to be tested with the known antibody for a certain period of time, and then mixing it with the latex particles sensitized by the corresp
The ways to enhance the immune response with antigen-expressing DNA are described. Two methods of nucleic acid immunization are described: (i) injection of sali
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