Isolation and purification of human metaphase immune cells
The metaphase immune cell population is the biological basis of maternal-fetal immune tolerance, and its composition is extremely special, mainly composed of sp
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Measurement of immune cell phagocytosis
The body has phagocytosis (phagocytosis) function of the cells called phagocytes, including monocyte macrophages and neutrophils two categories. Phagocytes phag
Monoclonal antibody identification test
The identification of monoclonal antibodies is similar to that of polyclonal antibodies, and the methods used are common; however, the identification of some of
Induction and isolation of human blood mononuclear macrophages
Macrophages (MΦ) are mature cells formed by the migration of blood monocytes into tissues, and are widely distributed in the spleen, liver, abdominal cavity, n
CFSE assay for human peripheral blood T cell expansion in vitro
CFSE detection of human peripheral blood T cell expansion in vitro refers to the use of CFSE to fluorescently label live cells, which can be used for the detect
B Phenotypic detection of cells
There are many membrane molecules on the surface of B cells, including cell surface differentiation antigen and model immunoglobulin (smlg). At different stages
Multi-parameter data acquisition and analysis experiments on leukocytes
Several software packages are now available for flow cytometry data analysis. Although each one has a different approach in processing the data, the basic strat
Preparation of monoclonal antibodies
Hybridoma cells are formed by fusing an immune animal B lymphocyte that produces a single antibody with myeloma cells by cell fusion techniques (e.g., PEG-induc
Isolation of peripheral blood single nucleated cells
Immune cell isolation techniques are developing in the direction of more and more "fine", immune cell subpopulation and even micro-immune cell isolation and ide