Detection of apoptosis in tumor cells by DNA degradation and fracture assay
DNA degradation fracture assay for detection of apoptosis in tumor cells can be used to (1) detect apoptosis in tumor cells and (2) detect the effect of drug tr
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Detection of Caspase-3 activity in apoptotic cells
The detection of Caspase-3 activity in apoptosis is mainly applied to (1) enzyme viability assay (2) apoptosis index assay.
Sample preparation experiments for DNA fluorescence staining
This experimental method was obtained from the official website of the Fourth Military Medical University
Preparation and analysis of cellular RNA content samples
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Apoptosis induced by staurosporine
Staurosporine is a potent inhibitor of protein kinase C and most other kinases, including tyrosine protein kinases. Directly inhibits topoisomerase II activity
Nunc Cell Factory
Prepare a cell suspension from the culture solution by injecting the suspension into each of the small chambers of the unit. Place with the long side down. Rota
4L Batch Stirred Suspension Culture
Cells were cultured on a small scale and then added to a culture aspiration flask pre-moderated and pre-filled with 5% CO2. The cell suspension was slowly agita
Experiments on Tec eukaryotic expression vector construction and its effect on cell signaling
Tec (Tyrosinekinaseexpressedinhepatocelluar carcinoma) is an important non-receptor tyrosine kinase, first cloned by Mano et al, from liver cancer tissue. The h
Calcein release assay to detect cytotoxic T-lymphocyte activity assay
The source of this experiment is the official website of the Fourth Military Medical University