Endothelial cell permeability assay
Changes in endothelial barrier function regulate vascular leakage into tissues to maintain homeostasis. Altered endothelial cell permeability is important in th
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Cell freezing and resuscitation experiments
Cell freezing and resuscitation can be (1) used for biological preservation, (2) used for stem cell research in medicine, and (3) used for passaging culture.
Mouse peritoneal macrophage culture experiment
Mouse peritoneal macrophage cultivation can (1) phagocytosis and removal of foreign substances; (2) secretion of bioactive substances; (3) to bionic fully degra
MTT assay for cytotoxicity and proliferation
The MTT assay is a common method for cell growth detection by MTT (chemical name 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide, trade name thiazolyl
Experimental purification of PCR products with dUTP
The incorporation of dUTP into the replicon during PCR amplification is one of the most efficient and widely used methods for purifying PCR products (Longoetal.
PCR amplification of high GC content fragments
The following program describes in detail the PCR amplification of high GC content antique fragments. This experiment was derived from PCR Laboratory Guide (Sec
Purification of sequencing reaction products
Purification of the sequencing reaction product prior to electrophoresis is essential to remove excess, unbound dye terminators. Centrifugation columns can be a
Primer extension experiment
Primer extension analysis was used to determine the location and quantify the amount of the 5'-end of a specific RNA. To end the labeled oligonucleotide hybridi
Genomic DNA purification from whole blood Experimental methods
The Wizard Genomic DNA Purification Kit for isolation of genomic DNA from whole blood (10 ml) is based on a four-step procedure. The first step of the purificat