PCR-based DNA library screening experimental methods
This experiment introduces the PCR-based DNA library screening method. This experiment is derived from PCR Laboratory Guide (Second Edition) by Seed Kang and Qu
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Colony PCR analysis of cloned recombinants experiments
The colony PCR analysis of cloned recombinants assay can be applied to (1) analyze whether colonies produced from transformed cells contain recombinant insert f
ribose cloning experiment
This scheme is just one way of treating PCR products with RNAase. There are many alternative and effective ways of purifying the PCR product to remove the prime
Experiment to amplify the 5' end of cDNA by classical RACE
This protocol is divided into three phases. Phase 1: reverse transcription to generate the cDNA template; phase 2: addition of poly(A) tail to the end of the fi
The 5' end of cDNA was amplified by the new RACE method.
This experimental program is divided into 6 stages. Stage 1: dephosphorylation of degraded RNA; Stage 2: uncapping of intact RNA; Stage 3: preparation of RNA ol
Analyzing substrates and products of ligation reactions by PCR
Synthetic DNA libraries generated by PCR can be digested enzymatically and ligated into a linearized expression vector system. In the scheme presented here, the
Hat Conversion RACE Experiment
The method presented here uses a biotinylated primer to help purify the first strand product. These purification techniques are interchangeable. Similarly, usin
Quality control experiments of a randomized peptide DNA library by PCR
In this scheme, a random polypeptide library encoded by a plasmid vector is analyzed by PCR. The library is obtained by transforming bacteria with the product o
Preparation experiments for cloning vectors
Many vectors and their derivatives have been developed for cloning PCR products, including the typical pBluescript-like vectors. These include the typical pBlue