Protein extraction experiments
Protein extraction experiments are basic operations for performing experimental analysis of proteins and can be used for (1) protein isolation (2) protein funct
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Protein expression, isolation and purification experiments
Protein expression, isolation and purification can: (1) explore and study the function of genes and the mechanism of gene expression regulation; (2) be used for
Quantitative methylation-specific PCR
Quantitative methylation specific PCR (qMSP) is a molecular biology method for detecting the degree of DNA methylation, which can determine the percentage of DN
Emulsion PCR experiment
In 2005, Margulies et al. published an article in Nature describing a fast and simple sequencing method based on an emulsion system for DNA amplification and a
PCR sequencing experiment
Since the introduction of dideoxynucleotide triphosphate (ddNTP) as a chain terminator by Sanger et al. (1975), the technology of DNA sequencing has developed r
Methylation-specific PCR
Methylation-specific PCR (MS-PCR) is a site-specific methylation detection technique, which is one of the most commonly used and highly accurate methods to stud
Polymerase Chain Reaction (PCR)
PCR technology can be used to (1) test whether infectious diseases are in a recessive or subclinical state; (2) effectively detect mutations in oncogenes and ac
PCR-based large fragment DNA synthesis assay
Chemical synthesis of DNA sequences provides a powerful tool for high level expression and functional studies of genes in heterologous systems. In recent years,
Real-time fluorescence quantitative PCR-FP
Real-time fluorescence quantitative PCR-FP