Analysis of N-terminal amino acids of proteins by dansulfonylation method
The reaction of α-amino group of protein with dansulfonyl chloride (DNS-Cl is a fluorescent substance) produces DNS-protein, which can be hydrolyzed to produce
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Expression and purification experiments of thioredoxin fusion protein
A gene fusion expression system using the E. coli trxA gene product-thioredoxin as a fusion partner is particularly suitable for the expression of high yield so
Electrophoretic separation and identification of proteins on cellulose acetate membranes
The isoelectric points of various proteins in mixed protein samples are different, and the amount of electrostatic charge carried by various proteins is differe
Salt chromatographic graded separation and gel chromatographic desalting of proteins
The purpose of this experiment is to understand the basic principles and operation of salting out graded separation of proteins; to understand the basic princip
Phosphate amino acid analysis experiment
It is of interest to identify phosphorylated amino acid residues in proteins. Phosphorylation occurs at serine, threonine, and methionine in proteins, and the i
Experiments to determine peak protein production
Protein is the material basis of life, without which there is no life. It is therefore a substance that is closely associated with life and with all forms of vi
The effect of substrate concentration on catalytic reaction rate and the determination of Mie constant Km and maximum reaction velocity Vmax
In this experiment, the Km value of sucrase was calculated by using the amount of products (glucose and fructose) formed by sucrase hydrolyzing different concen
Cyanogen bromide cleavage assay of calmodulin
This experiment describes the cyanogen bromide cleavage process of calmodulin. This experiment is from the Experimental Guide for Protein Purification and Ident
Serum lipoprotein agarose electrophoresis assay
After serum lipoproteins are stained with Sudan black B and electrophoresed in pH 8.6 barbiturate buffer with agarose, the lipoproteins can be divided into diff