3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
The spotted filter binding method is a rapid method for the initial determination of protein content. This method is particularly useful for screening useful fr
The use of gene fusion expression systems to express exogenous genes in E. coli has become increasingly popular. The reason for this is largely attributed to th
Batch chromatography is simple and fast, suitable for situations where protein resolution is not required, and an effective means of concentrating proteins. Sou
Gel filtration chromatography is an important protein purification technique, also known as size exclusion, gel exclusion, molecular sieve, or gel filtration ch
Ion exchange chromatography is the most widely used of the chromatographic means for purifying proteins. It has a high resolution for proteins, is easy to perfo
Dye-ligand chromatography is not really affinity chromatography because they are not natural ligands for the proteins to which they bind. However dye columns bi
Antibody affinity purification is one of the most effective methods in protein purification. This step alone often results in 1000 to 10,000-fold purification.
Interacting cloning (also known as expression cloning) is used to identify and clone proteins encoding proteins that interact with a target or 'bait' protein. T
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