Knowledge Base

Laboratory Protocols & SOPs Library

3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.

3228 articles
Laboratory Protocols & SOPs Library

Chemical degradation of fusion proteins

The use of gene fusion expression systems to express exogenous genes in E. coli has become increasingly popular. The reason for this is largely attributed to th

2025-06-10 03:34:01 Updated
Laboratory Protocols & SOPs Library

Immunopurification experiments

Antibody affinity purification is one of the most effective methods in protein purification. This step alone often results in 1000 to 10,000-fold purification.

2025-06-10 03:34:00 Updated
Laboratory Protocols & SOPs Library

Interaction cloning experiments

Interacting cloning (also known as expression cloning) is used to identify and clone proteins encoding proteins that interact with a target or 'bait' protein. T

2025-06-10 03:34:00 Updated
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