3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
The rate of sucrose hydrolysis was determined by measuring the amount of reducing sugars (glucose and fructose) produced, and in this experiment, the unit of su
When the dilute salt solution of DNA is heated to 80~100 ℃, the double helix structure will be disintegrated, the two strands will be separated, and a random c
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when
Grind the plant material in liquid nitrogen, make some cells broken, further make the plant cells lysed in lysis solution, precipitate protein with sodium aceta
The aim of this experiment was to further purify sucrase protein by column chromatography, using DEAE cellulose resin as an ion-exchange agent for hierarchical
Although most proteins bind approximately the same amount of dye per milligram, there is still a 10% or greater variation in the ability to bind dye between pro
The purpose of this experiment is to learn how to identify RNA by formaldehyde denaturing gel electrophoresis. Agarose gel electrophoresis is often used for the
This experiment describes the solubilization, refolding and ion exchange chromatography of inclusion body precipitation (σ32). This experiment was derived
Isoelectrofocusing, abbreviated as IEF or EF, was established in the 1960s as a means of separating and analyzing proteins, and has evolved rapidly over the pas
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