HVJ Liposomes and HVJ Packaging Vectors
This chapter describes the construction of a fusion-mediated vector based on inactivated H V J (hemagglutinating virus of Japan, also known as Sendaivirus). The
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
lipoplex and LPD nanoparticles for in vivo gene delivery
The core of gene therapy research lies in the development of suitable delivery systems. Although most clinical experiments currently utilize viral carrier syste
PEI Nanoparticles for Targeted Gene Delivery
P E I Due to its polycationic nature, it and negatively charged D N A molecules form nanoparticles through electrostatic interactions, which further help D N A
PEGylated nanoparticles of poly(L-lysine) and DNA
Nanoparticles of P E G-coated poly(L-lysine) and D N A are water-soluble, stable in saline and tissue fluids, transfect undivided cells (Liu et al. 2003), show
Water-soluble liposomes and lipopeptides for nucleic acid delivery
Water-soluble lipopolymers and lipopeptides are a class of nonviral gene vectors that combine the increased permeability of lipid materials to cell membranes by
Experiments on nanogene vectors based on polylactic acid and polyethylene glycol
Nanoparticles combining polylactic acid-hydroxyglycolic acid (PLGA) and polyethylene glycol (PEG) derivatives have been used as gene carriers for mucosal permea
High-throughput method for screening polymer transfection reagents
The development of a safe and effective gene delivery system is a prerequisite for the success of clinical gene therapy. Despite the high transfer efficiency of
Cationic polysaccharide assay for DNA delivery
Polycations are an effective class of non-viral gene delivery vectors. These vectors vary in molecular mass, chemical structure, polymer/DNA ratio, and molecula
Transposon-mediated delivery of small interfering RNAs: the "Sleeping Beauty" transposon experiment
A method for efficient delivery of pol III-driven SHRNA expression frames using the Sleeping Beauty (SB) transposon. Advantages of this system include its ease