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Laboratory Protocols & SOPs Library

3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.

3228 articles
Laboratory Protocols & SOPs Library

occult blood test (medicine)

Ferrous hemoglobin in o-toluidine zone method hemoglobin has peroxide E-like activity. It can catalyze H2O2 to release the new ecological O2 to oxidize the rece

2025-06-10 03:34:53 Updated
Laboratory Protocols & SOPs Library

Non-specific esterase staining assay

Nonspecific esterase (Nonspecific, esteras. NSE) staining method can be divided into three categories: neutral non-specific esterase, acidic non-specific estera

2025-06-10 03:34:53 Updated
Laboratory Protocols & SOPs Library

alkaline phosphatase staining assay

Alkaline phosphatase in cell tissues hydrolyzes the substrate (sodium naphthol a-monophosphate) in the action solution in an alkaline (PH 9.0-9.6) environment t

2025-06-10 03:34:53 Updated
Laboratory Protocols & SOPs Library

peroxidase staining assay

The peroxide E in the cell decomposes hydrogen peroxide to release O atoms, which oxidize benzidine and change it to benzidine orchid, causing the cell plasma t

2025-06-10 03:34:53 Updated
Laboratory Protocols & SOPs Library

Erythrocyte osmotic fragility test

This test is to determine the resistance of erythrocytes to different concentrations of hypotonic saline solution, this resistance is closely related to the rat

2025-06-10 03:34:53 Updated
Laboratory Protocols & SOPs Library

Cellular iron staining assay

Ferritin and ferritin (extracellular iron) in bone marrow and iron particles (intracellular iron) in naive erythrocytes interact with potassium ferrocyanide to

2025-06-10 03:34:53 Updated
Laboratory Protocols & SOPs Library

Sudan black staining experiment

Sudan black B (SB) is a fat-soluble dye, which can be dissolved in the lipid-containing structures in the cell plasma, so that the lipids in the cytoplasm appea

2025-06-10 03:34:53 Updated
Laboratory Protocols & SOPs Library

Periodic acid-Schiff's reaction experiment

The principle of PAS reaction to show sugars is due to the polysaccharides containing glycol groups, under the action of periodic acid, oxidation to produce ald

2025-06-10 03:34:53 Updated
Laboratory Protocols & SOPs Library

hemolytic test

This test uses the patient's erythrocytes to cause hemolysis to occur after incubation in their own serum (containing complement) at 37°C due to acid productio

2025-06-10 03:34:53 Updated
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