Cellular wound healing assay
The cellular wound healing assay is widely used and improved by researchers to study the role of various experimental conditions such as gene knockout and chemo
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Nitrate reductase activity assay (in vivo method)
Nitrate reductase activity assays can be used to: measure its efficiency in catalyzing the reduction of nitrate to nitrite in plants.
Nucleic acid sequence analysis experiment
Analysis against nucleic acid sequences is the process of searching for genes in nucleic acid sequences, identifying the location of genes and the location of f
Determination of chloroplast periplasmic integrity
Determination of chloroplast periplasmic membrane integrity can be used for (1) studies of the molecular mechanisms that promote photosynthesis and (2) detectio
Determination of chlorophyll content spectrophotometrically
Determination of chlorophyll content can be used to: (1) determine the photosynthetic capacity, i.e., health, of the plant; (2) as data for research to improve
Separation and fluorescence observation of chloroplasts
Tissue homogenization followed by suspension in isotonic media for differential centrifugation is a common method for separating organelles. The settling rate o
Observation of mitosis in plant cells
Mitosis, also known as indirect division, was first discovered in animals by W. Fleming (1882) and in plants by E. Strasburger (1880). Characterized by the pres
Determination of soluble sugar content in plants (anthrone method)
The determination of soluble sugars in fruits and vegetables can be used to: understand and identify the quality of fruits and vegetables.
Preparation of plant protoplasts
The preparation of plant protoplasts can be used for: (1) due to its ability to regenerate cell walls, carry out successive cell divisions and regenerate comple