SKIC2 Mouse mAb: FAQs for Research Use

SKIC2 Mouse mAb is a mouse-derived monoclonal antibody targeting the SKIC2 (Ski2) protein, a key RNA helicase component of the cytoplasmic SKI complex involved in RNA surveillance and 3′-to-5′ mRNA decay.

Product Name

SKIC2 Mouse mAb

Catalog Number

Ab349292

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

SKIC2 (Ski2, MTREX component, RNA helicase)

Reference Clone

Absea clone KC-540

Recommended Storage

Follow supplier-specified conditions; typically -20°C for long-term storage (verify with datasheet)

What Is SKIC2 and Why Is It Studied?

SKIC2 (also known as Ski2 or MTREX helicase) is a DExH-box RNA helicase that forms a core component of the cytoplasmic SKI complex. This complex cooperates with the cytoplasmic RNA exosome to mediate 3′-to-5′ degradation of mRNA, playing a critical role in mRNA surveillance pathways including nonsense-mediated decay (NMD) and other quality-control mechanisms. Disruption of SKIC2 function has been associated with Trichohepatoenteric syndrome (THES), a rare congenital disorder, underscoring its biological importance. Researchers study SKIC2 to understand cytoplasmic RNA turnover, gene expression regulation, and disease mechanisms linked to RNA metabolism defects.

Product Overview: SKIC2 Mouse mAb (Ab349292)

The SKIC2 Mouse mAb (catalog number Ab349292) is a mouse-derived monoclonal antibody developed against the human SKIC2 protein. It is based on the Absea clone KC-540. Monoclonal antibodies offer high specificity and lot-to-lot consistency, making them well-suited for reproducible detection of SKIC2 across experiments. Researchers should consult the official product datasheet for confirmed application compatibility, validated species reactivity, recommended dilutions, and storage instructions, as these details are authoritative and subject to update.

Recommended Applications and Usage Guidelines

Mouse monoclonal antibodies targeting SKIC2 are commonly evaluated in applications such as Western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). Specific validated applications for Ab349292 should be confirmed on the product datasheet. When setting up experiments, always include appropriate positive controls (cell lines or tissues with known SKIC2 expression) and negative controls (SKIC2 knockdown or knockout samples where available). Titrate the antibody for each application to determine the optimal working concentration for your system.

For Western blot, SKIC2 is a large protein (approximately 246 kDa); ensure SDS-PAGE gel percentage and transfer conditions are optimized for high-molecular-weight proteins.

Storage, Handling, and Stability

To maintain antibody performance, follow the storage conditions specified on the product datasheet and vial label. In general, monoclonal antibodies are stored at -20°C for long-term preservation and at 4°C for short-term use. Avoid repeated freeze-thaw cycles, which can reduce antibody activity; aliquoting the stock solution upon first use is recommended. Keep the antibody away from light and maintain sterile conditions when possible. Always check the expiration date and lot-specific documentation supplied with the product.

Ordering and Support

SKIC2 Mouse mAb (Ab349292) is available through Aladdin Scientific. To place an order, visit the product page on the Aladdin Scientific website or contact our customer service team. Bulk quantities, custom formats, or institutional pricing inquiries can be directed to our sales department. For technical questions regarding experimental setup, troubleshooting, or application validation, our technical support team is available to assist. Please have your catalog number (Ab349292) and lot number ready when contacting support for the fastest response.

Frequently asked questions

What is the catalog number for SKIC2 Mouse mAb at Aladdin Scientific?

The catalog number for SKIC2 Mouse mAb at Aladdin Scientific is Ab349292. This product is based on the Absea clone KC-540.

What applications is SKIC2 Mouse mAb (Ab349292) validated for?

Specific validated applications should be confirmed on the official Ab349292 product datasheet, as application data may be updated. Mouse monoclonal antibodies against SKIC2 are commonly assessed in Western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry. Please contact Aladdin Scientific technical support for the latest validation data.

What species reactivity does SKIC2 Mouse mAb (Ab349292) have?

Species reactivity information for Ab349292 should be verified on the current product datasheet available on the Aladdin Scientific website, as this information is lot-specific and subject to change. If reactivity against a non-human species is required, please contact our technical support team for guidance.

How should I store SKIC2 Mouse mAb (Ab349292) to maintain its activity?

Follow the storage instructions on the product datasheet and vial label. Generally, monoclonal antibodies are stored at -20°C for long-term use and 4°C for short-term use. Aliquot upon first use to avoid repeated freeze-thaw cycles, and protect from light.

What is the molecular weight of SKIC2, and does this affect Western blot setup?

SKIC2 is a large protein with a predicted molecular weight of approximately 246 kDa. For Western blot, use a low-percentage SDS-PAGE gel (e.g., 6–8%) and optimize transfer conditions (e.g., extended transfer time or wet transfer) to ensure efficient resolution and transfer of this high-molecular-weight protein.

How do I place an order for Ab349292 through Aladdin Scientific?

You can order Ab349292 directly through the Aladdin Scientific website by searching the catalog number, or by contacting our customer service team via email or phone. For institutional or bulk orders, please reach out to our sales department for pricing and availability.

References

1. Absea Biotechnology. KC-540 Clone Technical Information. Absea, Beijing.
2. Aladdin Scientific. SKIC2 Mouse mAb, catalog number Ab349292. Product Datasheet.
3. Halbach F, et al. Structural and functional analysis of the SKI complex. (Representative textbook-level reference — specific citation requires verification.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.